AI Article Synopsis
- The study assessed how tacrolimus affects the development of Walker 256 tumors in male Wistar rats by comparing treated and untreated groups.
- The experiment involved 40 rats divided into four groups: one with tumors only, one with tumors and tacrolimus, one with only tacrolimus, and a control group, and they were examined after 15 days.
- Results indicated that the tacrolimus-treated group had significantly higher creatinine and blood urea nitrogen levels, suggesting worsening kidney function, and post-mortem analysis showed severe tumor progression in this group.
Article Abstract
Purpose: To evaluate the development of Walker 256 tumor in male Wistar rats treated with tacrolimus using an experimental kidney tumor model.
Methods: 40 male Wistar rats were divided into four groups: Tumor group (TU) (n=10), Tacrolimus-Tumor group (TT) (n=10), Tacrolimus group (TC) (n=10) and Control group (C) (n=10). Treatment with tacrolimus was performed in groups TT and TC. Under anesthesia, the right kidney of each animal of TU and TT was accessed through a supraumbilical incision and inoculated with a 0.1 mL solution containing 2 x 10(6) tumor cells (Walker 256 carcinosarcoma tumor cells). Group TC was treated with a saline solution. All the animals of groups TC and TT were treated with tacrolimus (5mg/kg/day) by gavage for 15 days. TU group animals received saline by gavage for 15 days. On the 15th postoperative day, all animals were submitted to euthanasia and blood sampling for analysis of serum creatinine (Cr) and blood urea nitrogen (BUN). Abdominal gross examination was performed, the right kidney removed and prepared for histological analysis by hematoxylin-eosin staining. The resulting data were submitted to statistical analysis by ANOVA.
Results: Statistical significance was found when comparing creatinine level between groups TU, TT and TC -TT group culminated with a marked increased in creatinine levels (Cr=1.013 + or - 0.3028 mg/mL), TU group (Cr=0.5670 + or - 0.03536 mg/dL) P=0.00256, TC group (Cr =0.711 + or - 0.1653 mg/mL) P= 0.02832. Statistical significance was found when comparing BUN levels in TT group (71.32 + or - 17.14 mg/mL), compared with TU group (45.83 + or - 5.046 mg/dL), P=0.000318. There were no statistically significant differences between groups TT and TC (61.23 + or - 9.503 mg/mL) P=0.7242. Histological analysis showed a poor evolution in TT group with multiple foci of hemorrhage and cortical invasion by the Walker tumor.
Conclusion: The Tacrolimus-treated group developed a more aggressive tumor and a drug-related nephrotoxic effect.
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| http://dx.doi.org/10.1590/s0102-86502010000100020 | DOI Listing |
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