AI Article Synopsis
- The study explores an experimental method to isolate and analyze mesenchymal stem cells from rabbit fat obtained through lipectomy, suggesting fat as a viable source for tissue repair.
- After surgically removing and processing fat from New Zealand rabbits, the researchers cultured the obtained cells, leading to successful growth and high viability rates.
- Results indicated that stem cell extraction using lipectomy is effective, yielding significant quantities of viable stem cells for potential bioengineering applications.
Article Abstract
Purpose: To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering.
Methods: New Zealand rabbits (n = 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles.
Results: The fat total cells (CTF) were 1.62 x10(6) cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x10(6) cells/mL MSC. The same was done and after 20 days we quantified 4.28 x10(6) cells/mL MSC.
Conclusion: The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.
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| http://dx.doi.org/10.1590/s0102-86502010000100007 | DOI Listing |
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