Slow freezing of human oocytes is not yet a practical option. A new rapid freezing technique known as vitrification has recently been validated for use in assisted reproductive technologies. We propose to conduct a national trial aimed at validating this method in France. In theory this new approach can reduce the number of ovarian stimulations required for a give number of implantations. In addition, it has the potential to provide an alternative to embryo freezing, a procedure that raises the thorny issue of the fate of supernumerary embryos.
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Reprod Toxicol
January 2025
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, CEP: 60714-903, Fortaleza, CE, Brazil. Electronic address:
This study aimed to investigate, in vitro, the toxicity of WTA on ovarian follicles. Initially, a cytotoxicity assay was conducted using tumor and non-tumor cell lines to determine the ICof the WTA and validate its antitumor activity. Mouse ovaries were cultured in vitro (IVC) for 6 days in the presence of 1% dimethyl sulfoxide (DMSO), doxorubicin at 0.
View Article and Find Full Text PDFTheriogenology
January 2025
Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil. Electronic address:
This study aimed to investigate the changes induced by the culture system and the effect of ascorbic acid and resveratrol on collagen fibers, stromal cells, follicle growth and survival, as well as antioxidant enzyme activity in cultured bovine ovarian tissues. In experiment 1, bovine ovarian fragments were cultured in α-minimum essential medium (α-MEM) for 6 days. Before and after culturing, the fragments were fixed and processed to assess follicular morphology and diameters, stromal cell survival, collagen fibers, and glycosaminoglycans (GAGs).
View Article and Find Full Text PDFJ Assist Reprod Genet
January 2025
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, Sassari, Italy.
Purpose: This study aimed to evaluate the effectiveness of single versus group culture strategies for cumulus-oocyte complexes (COCs) derived from early antral follicles (EAFs), with the goal of optimizing culture conditions to increase oocyte availability for assisted reproductive technologies.
Methods: COCs isolated from EAFs (350-450 µm) from sheep ovaries were cultured in TCM199 medium supplemented with 0.15 µg/mL Zn as zinc sulfate, 10 IU/mL FSH, 10 ng/mL estradiol, 50 ng/mL testosterone, 50 ng/mL progesterone, and 5 µM Cilostamide.
Syst Biol Reprod Med
December 2025
Department of Mathematics and Computer Science, Laboratory of Analysis, Modeling and Simulation, Faculty of Sciences Ben M'sik, Hassan II University of Casablanca, Casablanca, Morocco.
Infertility has emerged as a significant public health concern, with assisted reproductive technology (ART) is a last-resort treatment option. However, ART's efficacy is limited by significant financial cost and physical discomfort. The aim of this study is to build Machine learning (ML) decision-support models to predict the optimal range of embryo numbers to transfer, using data from infertile couples identified through literature reviews.
View Article and Find Full Text PDFEnviron Res
January 2025
Guangxi Key Laboratory of Environmental Exposomics and Entire Lifecycle Health, School of Public Health, Guilin Medical University, Guilin, 541199, PR China. Electronic address:
Pentachlorophenol (PCP), a persistent organic pollutant, has endocrine disrupting properties and there may be a link between its exposure and reproductive outcomes. In this study, we assessed the relationship of PCP exposure levels with ovarian reserve markers and reproductive health outcomes in women (N = 656) undergoing in vitro fertilization (IVF). PCP concentrations were determined in urine (n = 1,968; repeated measures) and follicular fluid samples (n = 603).
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