Xylanases have important applications in industry. Immobilization and stabilization of enzymes may allow their reuse in many cycles of the reaction, decreasing the process costs. This work proposes the use of a rational approach to obtain immobilized commercial xylanase biocatalysts with optimized features. Xylanase NS50014 from Novozymes was characterized and immobilized on glyoxyl-agarose, agarose-glutaraldehyde, and agarose-amino-epoxy support and on differently activated chitosan supports: glutaraldehyde-chitosan, glyoxyl-chitosan, and epoxy-chitosan. Two different chitosan matrices were tested. The best chitosan derivative was epoxy-chitosan-xylanase, which presented 100% of immobilization yield and 64% of recovered activity. No significant increase on the thermal stability was observed for all the chitosan-enzyme derivatives. Immobilization on glyoxyl-agarose showed low yield immobilization and stabilization degrees of the obtained derivative. The low concentration of lysine groups in the enzyme molecule could explain these poor results. The protein was then chemically modified with ethylenediamine and immobilized on glyoxyl-agarose. The new enzyme derivatives were 40-fold more stable than the soluble, aminated, and dialyzed enzyme (70 degrees C, pH 7), with 100% of immobilization yield. Therefore, the increase of the number of amine groups in the enzyme surface was confirmed to be a good strategy to improve the properties of immobilized xylanase.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12010-009-8897-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ultrasensitive point-of-care multiplex diagnosis for influenza virus based robust quantum dot microsphere-lateral flow immunoassay.
Biosens Bioelectron
January 2025
Key Lab for Special Functional Materials of Ministry of Education, and School of Nanoscience and Materials Engineering, Henan University, 475004, Kaifeng, China. Electronic address:
Influenza A virus (IAV) and influenza B virus (IBV) with similar symptoms of infection caused a serious disease burden and economic losses in annual epidemic season, so it is important to quickly and accurately detect and distinguish between IAV and IBV during influenza season. Herein, the quantum dot microspheres (QDMS) were synthesized and applied to lateral flow immunoassays (LFIA), and a point-of-care (POC) biosensor that can discriminately and simultaneously diagnose IAV and IBV within 10 min was established. A double-sandwich QDMS nanotags was synthesized by immobilizing hydrophobic quantum dots (QDs) with chemical bonding method on a silica sphere template with an outer silica shell protection showed excellent stability and high fluorescence.
View Article and Find Full Text PDFComputational Modeling of Electrocatalysts for CO Reduction: Probing the Role of Primary, Secondary, and Outer Coordination Spheres.
Acc Chem Res
January 2025
The Department of Chemistry, State University of New York at Binghamton, Binghamton, New York 13902, United States.
ConspectusIn the search for efficient and selective electrocatalysts capable of converting greenhouse gases to value-added products, enzymes found in naturally existing bacteria provide the basis for most approaches toward electrocatalyst design. Ni,Fe-carbon monoxide dehydrogenase (Ni,Fe-CODH) is one such enzyme, with a nickel-iron-sulfur cluster named the C-cluster, where CO binds and is converted to CO at high rates near the thermodynamic potential. In this Account, we divide the enzyme's catalytic contributions into three categories based on location and function.
View Article and Find Full Text PDFCarbon quantum dot-anchored polyaniline on electrospun carbon nanofibers as freestanding electrodes for symmetric solid-state supercapacitors.
Dalton Trans
January 2025
Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, School of Physics and Electronic Engineering, Harbin Normal University, Harbin 150025, People's Republic of China.
A binder-free and freestanding electrode was designed by uniformly immobilizing carbon quantum dot (CQD)-anchored polyaniline (PANI) heterostructures onto electrospun carbon nanofibers (CNFs) a facile hierarchical assembly process. The fabricated freestanding CNF/PANI/CQD electrode exhibits a unique three-dimensional (3D) network nanostructure, which accelerates ion migration between the interior and surface of the electrode, thereby enhancing its charging and discharging performance. Moreover, the functional groups on the surface of CQDs could anchor PANI through possible chemical bonding, which not only improves the stability of the PANI/CQD heterojunction but also creates an additional conductive channel for the PANI polymer.
View Article and Find Full Text PDFOptimized Synthesis and Stabilization of Superparamagnetic Iron Oxide Nanoparticles for Enhanced Biomolecule Adsorption.
ACS Omega
January 2025
Federal University of Espírito Santo, Av Marechal Campos 1468, Vitória, ES 29.040 090, Brazil.
Monodisperse and colloidally stable magnetic iron oxide nanoparticles have been developed for diverse biotechnology applications. Although promising for the adsorption of organic molecules, the low density of adsorption sites in these nanoparticles has been a significant challenge. In this study, an optimized factorial design with response surface methodology (RSM) was employed to produce small Superparamagnetic Iron Oxide Nanoparticles (SPIONs) stabilized with tetraethoxysilane (TEOS).
View Article and Find Full Text PDFMitigating cadmium contamination in soil using Biochar, sulfur-modified Biochar, and other organic amendments.
Int J Phytoremediation
January 2025
College of Engineering, Agriculture Aviation Innovation Lab, South China Agriculture University, Guangzhou, China.
Biochar is a novel approach to remediating heavy metal-contaminated soil. Using various organic amendments like phyllosilicate-minerals (PSM), compost, biochar (BC) and sulfur-modified biochar (SMB), demonstrates superior adsorption capacity and stability compared to unmodified biochar (BC). The adsorption mechanisms of SMB are identified for its potential to increase soil-pH and reduce available cadmium (Cd).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!