Time-course of mechanical changes of the rat aorta following chronic beta-blocker treatment.

Introduction: The mechanical properties of the aorta play an important role in arterial homeostasis and constitute a prognostic factor in cardiovascular disease. This study determined the time-course of mechanical changes of the thoracic aorta following prolonged beta (beta)-blocker treatment.

Methods: Sixty-six healthy male Wistar rats were randomized to 4 groups. Group A was divided into subgroups A1 (n=6), A2 (n=6), and A3 (n=6), with animals receiving only water. In groups B (n=16), C (n=16), and D (n=16), propranolol was added to the drinking water (100 mg/kg/day). Animals of groups A1 and B, A2 and C, and A3 and D were sacrificed after 1, 2, and 3 months. The effect of beta-blockade was assessed by heart rate changes in response to isoproterenol infusion. The thoracic aorta was excised and submitted to mechanical testing. Regression analysis was performed to evaluate the relationship between elastic modulus and stress for low (part I), physiologic (part II), and high (part III) stresses.

Results: Data from subgroups A1, A2, and A3 were pooled together and were used as a control. Differences were found in the regression parameters of parts II and III between the propranolol-treated groups and controls, indicating that the aorta was stiffer in propranolol-treated rats compared to controls at physiologic stresses, and at physiologic and high strains. Changes developed progressively with the duration of treatment. No differences were found in the regression parameters of part I, indicative of non-varying elastic modulus, i.e. stiffness, at low stresses and strains.

Conclusions: Chronic blockade of beta-adrenergic receptors induces changes in the mechanical properties of the thoracic aorta. Aortic stiffening in response to beta-blocker treatment may be of great clinical significance.