AI Article Synopsis
- - The study focuses on the type VI secretion system (H1-T6SS) of Pseudomonas aeruginosa and its functionally defined secretome, which reveals three key substrates (Tse1-3 proteins) regulated alongside the secretion machinery.
- - Tse2, one of the substrates, acts as a toxin within bacteria, inhibiting growth when present inside cells but not affecting eukaryotic cells when secreted.
- - It was found that Tse2 gives Pseudomonas aeruginosa a competitive edge over strains without immunity, showcasing a link between the T6SS and bacteriophage structures in their evolutionary context.
Article Abstract
The functional spectrum of a secretion system is defined by its substrates. Here we analyzed the secretomes of Pseudomonas aeruginosa mutants altered in regulation of the Hcp Secretion Island-I-encoded type VI secretion system (H1-T6SS). We identified three substrates of this system, proteins Tse1-3 (type six exported 1-3), which are coregulated with the secretory apparatus and secreted under tight posttranslational control. The Tse2 protein was found to be the toxin component of a toxin-immunity system and to arrest the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cells; however, it provided a major growth advantage for P. aeruginosa strains, relative to those lacking immunity, in a manner dependent on cell contact and the H1-T6SS. This demonstration that the T6SS targets a toxin to bacteria helps reconcile the structural and evolutionary relationship between the T6SS and the bacteriophage tail and spike.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831478 | PMC |
| http://dx.doi.org/10.1016/j.chom.2009.12.007 | DOI Listing |
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