AI Article Synopsis

  • Stem cell therapies have potential for repairing heart tissue, and researchers developed dual-reporter mouse embryonic stem (mES) cell lines to study their effectiveness.
  • One specific clonal cell line, Ncx-1-43LUC mES cells, showed a significant increase in a reporter gene when induced to differentiate into heart cells, indicating stronger cardiac development.
  • Transplanting these differentiated cells into neonatal mouse hearts demonstrated sustained cardiac differentiation over weeks, while undifferentiated cells did not show this capability, highlighting the importance of pre-differentiation for successful heart cell therapy.

Article Abstract

Stem cell therapies offer the potential for repair and regeneration of cardiac tissue. To facilitate evaluation of stem cell activity in vivo, we created novel dual-reporter mouse embryonic stem (mES) cell lines that express the firefly luciferase (LUC) reporter gene under the control of the cardiac sodium-calcium exchanger-1 (Ncx-1) promoter in the background of the 7AC5-EYFP mES cell line that constitutively expresses the enhanced yellow fluorescent protein (EYFP). We compared the ability of recombinant clonal cell lines to express LUC before and after induction of cardiac differentiation in vitro. In particular, one of the clonal cell lines (Ncx-1-43LUC mES cells) showed markedly enhanced LUC expression (45-fold increase) upon induction of cardiac differentiation in vitro. Further, cardiac differentiation in these cells was perpetuated over a period of 2-4 weeks after transplantation in a neonatal mouse heart model, as monitored by noninvasive bioluminescence imaging (BLI) and confirmed via postmortem immunofluorescence and histological assessments. In contrast, transplantation of undifferentiated pluripotent Ncx-1-43LUC mES cells in neonatal hearts did not result in detectable levels of cardiac differentiation in these cells in vivo. These results suggest that prior induction of cardiac differentiation in vitro enhances development and maintenance of a cardiomyocyte-like phenotype for mES cells following transplantation into neonatal mouse hearts in vivo. We conclude that the Ncx-1-43LUC mES cell line is a novel tool for monitoring early cardiac differentiation in vivo using noninvasive BLI.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121874PMC
http://dx.doi.org/10.1089/scd.2009.0308DOI Listing

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