AI Article Synopsis
- Serotonin (5-HT) plays a key role in the maturation and transport of oocytes in bivalves, specifically triggering germinal vesicle breakdown (GVBD) and facilitating movement through cilia.
- The study focused on the characterization and cloning of the 5-HT receptor from the Japanese scallop, revealing a new receptor (5-HT(py)) with similarities to those found in other species, indicating a shared evolutionary background.
- Results showed that the 5-HT(py) gene is expressed in the scallop’s nervous system and tissues, with increased expression in ovarian tissue due to estradiol-17beta (E(2)), suggesting its involvement in oocyte maturation and sperm transport.
Article Abstract
Serotonin (5-hydroxytriptamin, 5-HT) triggers germinal vesicle breakdown (GVBD) of oocytes and the transporting of the mature oocyte through the gonoduct via cilia motility in bivalves. The 5-HT receptor in the oocyte membrane of the Japanese scallop, Patinopecten yessoensis, has been pharmacologically characterized as a mixed profile of 5-HT(1)/5-HT(2) and is induced by estradiol-17beta (E(2)). Here we report the isolation, cloning, and tissue expression of the 5-HT receptor from the gonad of the Japanese scallop. A full-length cDNA (1818 bp) encoding a putative 5-HT receptor (5-HT(py)) of 454 amino acid residues was isolated from the ovary and shared 53.3% and 40.2% homology with the Aplysia 5-HT(1ap) and mouse 5-HT(1A), respectively. The 5-HT(py) sequence possessed typical characteristics of 5-HT(1), including seven transmembrane domains, a long third inner loop, and a short fourth inner terminal. Phylogenetic analysis suggested that 5-HT(py) was classified into the 5-HT(1) subtype as well as other invertebrate 5-HT(1) receptors. Semi-quantitative RT-PCR showed the expression of the 5-HT(py) gene in both the nervous system and peripheral tissues and the induction of expression by E(2) in the ovarian tissue. In situ hybridization revealed a strong 5-HT(py) signal in the oocytes, spermatids, and ciliary epithelium of the gonoducts in the ovary and testis. These results suggest that the effects of 5-HT on the induction of oocyte maturation, sperm motility, and transport of mature oocytes and sperm through the ciliated epithelium of the gonoducts are mediated by 5-HT(py).
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| http://dx.doi.org/10.1016/j.ygcen.2010.01.014 | DOI Listing |
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