AI Article Synopsis
- The study aimed to assess the quality and functionality of hematopoietic progenitor cells from cord blood stored at a specific bank in Colombia.
- After thawing and processing 20 samples, researchers found that there was a significant drop in the percentage of viable progenitor cells, but they still managed to yield a reasonable number of cell colonies for both red and white blood cells.
- Despite a notable decrease in cell viability after thawing, the results indicated that the functional capacity of these cells was comparable to results from other established methods.
Article Abstract
Objective: To evaluate the viability and functional capacity of hematopoietic progenitor cells from cord blood samples cryopreserved at the Banco de Células Stem de Colombia.
Methods: After thawing and centrifugation of 20 samples, viable white blood cells were numbered by the trypan blue method and CD34(+)CD45(+dim) hematopoietic progenitor cells were numbered by flow cytometry. Clonogenic assays also tested the functional capacity of viable CD34(+)CD45(+dim) cells.
Results: The median rates of viable CD34(+)CD45(+dim) cells were 99.6% before freezing and 73.0% after thawing (P<0.001). The 20 cultures yielded a median of 12 cells with a lineage of red cells, 17.5 cells with a lineage of white cells, and 10 cells with a mixed lineage.
Conclusion: Although the rate of viable CD34(+)CD45(+dim) cells was decreased by 26.6% after thawing by the method we used, the numbers of CD34(+)CD45(+dim) cells that formed colonies were similar to those obtained by other published methods.
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| http://dx.doi.org/10.1016/j.ijgo.2009.11.014 | DOI Listing |
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