By means of real-time RT-PCR and immunofluorscent staining combined with confocal microscopy we show for the first time the expression ofmRNA and protein ofthe cold/menthol-sensitive cationic channel, TRPM8, in the smooth muscle cells (SMC) from the epididimal and prostatic portions of the rat vas deferens. Expression of TRPM8 mRNA correlated with the expression ofmRNA for androgen receptor (AR): orchidectomy of the animals resulted in the enhancement of the expression of both mRNAs, which likely reflects specific for the vas deferens compensatory response to the decreasing levels of circulating androgens. TRPM8 protein in the SMC from both parts of the vas deferens primarily localized outside the plasma membrane (PM), however, in the SMC from prostatic portion we observed higher TRPM8 protein targeting specifically the endoplasmic reticulum and PM, where it can form functional cold/menthol-sensitive cationic channel capable of modulating contractile activity of the smooth muscle.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
6-Nitrodopamine is an endogenous mediator of rat seminal vesicles contractility.
Andrology
January 2025
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil.
Background: 6-Nitrodopamine (6-ND) released from rat vas deferens acts an endogenous modulator of vas deferens contractility.
Objectives: To investigate whether rat isolated seminal vesicles (RISV) releases 6-ND, the mechanisms involved in the release, and the modulatory role of 6-ND on tissue contractility.
Methods: Rat seminal vesicles were removed and placed in Krebs-Henseleit's solution at 37°C for 30 min, and an aliquot was used to analyze the concentrations of 6-ND, dopamine, noradrenaline, and adrenaline by liquid chromatography with tandem mass spectrometry (LC-MS/MS).
Leptin action on ARC-PVN neural circuit regulates ejaculation behavior by altering sympathetic neuroplasticity.
Andrology
January 2025
Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Background: Although some studies have revealed the close relationship between leptin and premature ejaculation in clinical practice, whether and how leptin participates in the regulation of ejaculatory behaviors are still unknown.
Objective: To explore the role of leptin on ejaculatory behaviors and its underlying mechanism.
Materials And Methods: Copulation behavior tests were performed after acute and chronic leptin administration at peripheral and central levels.
Anatomical recognition of dissection layers, nerves, vas deferens, and microvessels using artificial intelligence during transabdominal preperitoneal inguinal hernia repair.
Hernia
December 2024
Department of Surgery, Tsudanuma Central General Hospital, 1- 9-17 Yatsu, Narashino, Japan.
Purpose: In laparoscopic inguinal hernia surgery, proper recognition of loose connective tissue, nerves, vas deferens, and microvessels is important to prevent postoperative complications, such as recurrence, pain, sexual dysfunction, and bleeding. EUREKA (Anaut Inc., Tokyo, Japan) is a system that uses artificial intelligence (AI) for anatomical recognition.
View Article and Find Full Text PDFIncidental finding of duplication of vas deferens in inguinal hernia: a case report of rare anomaly.
J Surg Case Rep
December 2024
Maharajgunj Medical Campus, Tribhuvan University, Institute of Medicine, Kathmandu 44600, Nepal.
Duplication of the vas deferens is a rare congenital anomaly, with an incidence of ~0.05% in the general population. It is typically discovered incidentally during surgeries involving the spermatic cord, such as inguinal hernia repair.
View Article and Find Full Text PDFPreserving one artery shortens the surgical time and does not affect the efficacy of microsurgical subinguinal varicocelectomy: preliminary findings from a retrospective study.
BMC Urol
December 2024
Department of Urology, 920th Hospital of Joint Logistic Support Force, Kunming, 650000, China.
Background: To analyze the safety and efficacy of microsurgical subinguinal varicocelectomy(MSV) performed with and without preservation of all testicular arteries and lymphatic system.
Methods: All of the 98 patients with varicocele who underwent MSV were included in the analysis. Fifty-eight male patients surgically underwent MSV with preservation of all testicular arteries and lymphatic system(Group 1).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!