Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length (FL) cDNAs (rice FOX Arabidopsis lines) using a gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS)-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candidate lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Arabidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries (LOB) Domain (LBD)/Asymmetric Leaves2-like (ASL) gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/mp/ssp069 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Article Synopsis
- Background: The study focused on the effectiveness of tranexamic acid in preventing bleeding and reducing the need for platelet transfusions in patients with blood cancers receiving intensive treatment.
- Methods: An international trial (TREATT) involved 616 adults under chemotherapy or stem-cell transplant, who were randomly assigned to receive either tranexamic acid or a placebo while monitored for bleeding and platelet recovery.
- Findings: Out of 1736 screened patients, 597 were included in the final analysis, revealing demographic details and outcomes related to bleeding events and mortality rates among the two groups.
A Randomized Crossover Trial of Acceptability of Quadruple-Fortified Salt in Women and their Households in Southern India.
J Nutr
October 2024
Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States; Joan Klein Jacobs Center for Precision Nutrition and Health, Cornell University, Ithaca, NY, United States; Division of Epidemiology, Department of Population Health Sciences, Weill Cornell Medicine, New York, NY, United States; Division of Nutrition, St. John's Research Institute, Bengaluru, Karnataka, India. Electronic address:
Background: Double-fortified salt (DFS; iron, iodine) improved iron status in randomized trials and was incorporated into India's social safety net programs, suggesting opportunities to address other micronutrient deficiencies.
Objectives: To evaluate the acceptability of quadruple-fortified salt (QFS; iron, iodine, folic acid, and vitamin B) in women and their households, using a randomized crossover trial design and triangle tests.
Methods: Women 18-49 y (n = 77) and their households were randomly assigned to receive QFS or DFS in a randomized crossover design over a 3-wk period (week 1: QFS/DFS, week 2: iodized salt, week 3: DFS/QFS).
Large-scale exome array summary statistics resources for glycemic traits to aid effector gene prioritization.
Wellcome Open Res
October 2023
MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Institute of Metabolic Science, Cambridge Biomedical Campus, Cambridge, CB2 0QQ, UK.
Article Synopsis
- Genome-wide association studies have found numerous genetic loci linked to glycemic traits, but connecting these loci to specific genes and biological pathways remains a challenge.
- Researchers conducted meta-analyses of exome-array studies across four glycemic traits, analyzing data from over 144,000 participants, which led to the identification of coding variant associations in more than 60 genes.
- The study revealed significant pathways related to insulin secretion, zinc transport, and fatty acid metabolism, enhancing understanding of glycemic regulation and making data available for further research.
Yeast EndoG prevents genome instability by degrading extranuclear DNA species.
Nat Commun
September 2024
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, USA.
In metazoans mitochondrial DNA (mtDNA) or retrotransposon cDNA released to cytoplasm are degraded by nucleases to prevent sterile inflammation. It remains unknown whether degradation of these DNA also prevents nuclear genome instability. We used an amplicon sequencing-based method in yeast enabling analysis of millions of DSB repair products.
View Article and Find Full Text PDFHaloTag as a substrate-based macroautophagy reporter.
Proc Natl Acad Sci U S A
August 2024
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037.
Macroautophagy is a conserved cellular degradation pathway that, upon upregulation, confers resilience toward various stress conditions, including protection against proteotoxicity associated with neurodegenerative diseases, leading to cell survival. Monitoring autophagy regulation in living cells is important to understand its role in physiology and pathology, which remains challenging. Here, we report that when HaloTag is expressed within a cell of interest and reacts with tetramethylrhodamine (TMR; its ligand attached to a fluorophore), the rate of fluorescent TMR-HaloTag conjugate accumulation in autophagosomes and lysosomes, observed by fluorescence microscopy, reflects the rate of autophagy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!