Rapid and specific method for evaluating Streptomyces competitive dynamics in complex soil communities.

Appl Environ Microbiol

Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, Minnesota 551081, USA.

Published: March 2010

Quantifying target microbial populations in complex communities remains a barrier to studying species interactions in soil environments. Quantitative PCR (qPCR) assays were developed for quantifying pathogenic Streptomyces scabiei and antibiotic-producing Streptomyces lavendulae strains in complex soil communities. This assay will be useful for evaluating the competitive dynamics of streptomycetes in soil.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837995PMC
http://dx.doi.org/10.1128/AEM.02320-09DOI Listing

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