Objective: To compare different Mycobacterium tuberculosis antigens in enzyme-linked immunospot assay (ELISPOT) for the auxiliary diagnosis of active tuberculosis.
Methods: The peripheral blood mononuclear cells (PBMC) from patients with tuberculosis and controls were co-cultured with the following antigens: purified protein derivative (PPD), early secretory antigenic target-6 (ESAT-6) and early secretory antigenic target-6/culture filtrate protein-10 fusion protein (ESAT-6/CFP-10). Spot forming cells (SFC) were enumerated by ELISPOT.
Results: PPD-ELISPOT, E/C-ELISPOT and ESAT-6-ELISPOT showed significantly higher SFC counts in active tuberculosis [255(93-526), 148(40-354) and 28(10-116) respectively] as compared to the controls [10(5-41), 10(0-20) and 5(0-15) respectively], u values were 1479.5, 1390.5 and 2510.5 respectively, all P < 0.01. Compared with the sensitivity of ESAT-6-ELISPOT (62.1%), that of E/C-ELISPOT and PPD-ELISPOT was higher (90.3% and 84.8%), chi(2) = 17.496 and 28.541, all P < 0.01. Compared with the specificity of PPD-ELISPOT (68.9%), that of E/C-ELISPOT and ESAT-6-ELISPOT was also higher (84.4% and 88.9%), chi(2) = 6.807 and 10.808, P < 0.05 and P < 0.01 respectively.
Conclusions: E/C-ELISPOT is a promising approach to the auxiliary diagnosis of tuberculosis, but its specificity could be affected by latent tuberculosis infection.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Assessing the Diagnostic Value of Mean Monocyte Volume and Hematological Parameters in Predicting Dengue Fever: A Cross-Sectional Analysis.
Cureus
December 2024
Department of Hematology, School of Medical Sciences, Universiti Sains Malaysia, Kota Bharu, MYS.
Article Synopsis
- This study addresses the difficulty of distinguishing dengue fever (DF) from other viral infections by exploring the diagnostic potential of hematological parameters, particularly mean monocyte volume (MMV), in Malaysia.
- A cross-sectional study analyzed 162 patients with suspected DF, confirming diagnoses through specialized blood tests and measuring various blood parameters using advanced hematology analyzers.
- Results indicated significant differences in certain blood counts between DF and non-DF patients, with monocyte percentage showing promise as a moderate predictor for DF, although MMV did not correlate, suggesting areas for future research.
A pan-immune panorama of bacterial pneumonia revealed by a large-scale single-cell transcriptome atlas.
Signal Transduct Target Ther
January 2025
Experimental Research Center, Capital Institute of Pediatrics, Beijing, 100020, P.R. China.
Bacterial pneumonia is a significant public health burden, contributing to substantial morbidity, mortality, and healthcare costs. Current therapeutic strategies beyond antibiotics and adjuvant therapies are limited, highlighting the need for a deeper understanding of the disease pathogenesis. Here, we employed single-cell RNA sequencing of 444,146 bronchoalveolar lavage fluid cells (BALFs) from a large cohort of 74 individuals, including 58 patients with mild (n = 22) and severe (n = 36) diseases as well as 16 healthy donors.
View Article and Find Full Text PDFS100 calcium-binding protein A8 exacerbates deep vein thrombosis in vascular endothelial cells.
Sci Rep
January 2025
Vascular Gland Surgery, The First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, Hebei, China.
Previous studies highlighting the pivotal function of the S100A8 protein have shown that inflammation and vascular endothelial harm play a major role in deep vein thrombosis (DVT) development, as evidenced by earlier studies highlighting the pivotal function of the S100 calcium-binding protein A8 (S100A8). Therefore, we aimed to establish a connection between S100A8 and DVT and investigate the role of S100A8 in DVT development. Blood specimens were taken from 23 patients with DVT and 31 controls.
View Article and Find Full Text PDFEpidemiological Study on Humans, Animals and Snails for Schistosomiasis in Two Endemic Municipalities Nearing Elimination in Bohol, the Philippines.
Acta Parasitol
January 2025
World Health Organization Regional Office for Southeast Asia, New Delhi, India.
Purpose: A comprehensive survey was conducted to assess the prevalence of Schistosoma japonicum infection in humans, water buffaloes, and snails in the two endemic municipalities of Talibon and Trinidad in Bohol, Philippines, which are nearing elimination.
Methods And Results: Human stool and blood samples were collected from barangays with snail breeding sites, and results showed higher positivity rates using the rSjTPx-1-ELISA compared to the Kato-Katz technique. Human stool examination for showed a 0.
Evaluation of the protective role of resveratrol on LPS-induced septic intestinal barrier function via TLR4/MyD88/NF-κB signaling pathways.
Sci Rep
January 2025
Department of Critical Care Medicine, Tongde Hospital of Zhejiang Province, #234 Gucui Road, Hangzhou, 310012, Zhejiang, People's Republic of China.
The intestinal barrier function is a critical defense mechanism in the human body, serving as both the primary target and initiating organ in cases of sepsis. Preserving the integrity of this barrier is essential for preventing complications and diseases, including sepsis and mortality. Despite this importance, the impact of resveratrol on intestinal barrier function remains unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!