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Preferential expression and immunogenicity of HIV-1 Tat fusion protein expressed in tomato plant. | LitMetric

Preferential expression and immunogenicity of HIV-1 Tat fusion protein expressed in tomato plant.

Transgenic Res

Department of Molecular and Cellular Biology¸ Molecular and Cellular Biology Laboratory, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Misuho-cho, Mizuho-ku, Nagoya, Aichi, 467-8601, Japan.

Published: October 2010

AI Article Synopsis

Article Abstract

HIV-1 Tat plays a major role in viral replication and is essential for AIDS development making it an ideal vaccine target providing that both humoral and cellular immune responses are induced. Plant-based antigen production, due to its cheaper cost, appears ideal for vaccine production. In this study, we created a plant-optimized tat and mutant (Cys30Ala/Lys41Ala) tat (mtat) gene and ligated each into a pBI121 expression vector with a stop codon and a gusA gene positioned immediately downstream. The vector construct was bombarded into tomato leaf calli and allowed to develop. We thus generated recombinant tomato plants preferentially expressing a Tat-GUS fusion protein over a Tat-only protein. In addition, plants bombarded with either tat or mtat genes showed no phenotypic difference and produced 2-4 microg Tat-GUS fusion protein per milligram soluble plant protein. Furthermore, tomato extracts intradermally inoculated into mice were found to induce a humoral and, most importantly, cellular immunity.

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Source
http://dx.doi.org/10.1007/s11248-009-9358-9DOI Listing

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