Objective: To develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha.
Method: The determination was carried out on an HC-C18 column elnted with acetonitrile-water (7:93) as mobile phase, and the detection wavelength was set at 238 nm.
Result: There is a good linearity in the range 22.08-552 mg L(-1) of scandoside methyl ester and the average recovery is 97.7% (n = 6), RSD 0.72%.
Conclusion: This method is convenient, quick, and is applicable to the quality control of H. chrysotricha.
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