The purpose of this work is to demonstrate the functionality and performance of a PSF-based geometric distortion correction for high-field functional animal EPI. The EPI method was extended to measure the PSF and a postprocessing chain was implemented in Matlab for offline distortion correction. The correction procedure was applied to phantom and in vivo imaging of mice and rats at 9.4T using different SE-EPI and DWI-EPI protocols. Results show the significant improvement in image quality for single- and multishot EPI. Using a reduced FOV in the PSF encoding direction clearly reduced the acquisition time for PSF data by an acceleration factor of 2 or 4, without affecting the correction quality.
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http://dx.doi.org/10.1155/2009/946271 | DOI Listing |
We theoretically demonstrate that ponderomotive interactions near the electron cross-over can be used for aberration correction in ultrafast electron microscopes. Highly magnified electron shadow images from SiN thin films are utilized to visualize the distortions induced by spherical aberrations. Our simulations of electron-light interactions indicate that spherical aberrations can be compensated resulting in an aberration-free angle of 8.
View Article and Find Full Text PDFStitching interferometry is essential for X-ray mirror metrology where lateral distortion and pixel size are critical factors influencing its accuracy. Simulations and experiments reveal that 1% error in pixel size causes a 2% deviation in the radius of curvature. After correcting pixel size, the stitching profile error of an elliptical mirror reduces from 2 μm to 250 nm (peak-to-valley).
View Article and Find Full Text PDFBMC Bioinformatics
January 2025
Department of Radiology, University of Minnesota, Minneapolis, MN, 55455, USA.
RNA sequencing (RNA-seq) is the conventional genome-scale approach used to capture the expression levels of all detectable genes in a biological sample. This is now regularly used for population-based studies designed to identify genetic determinants of various diseases. Naturally, the accuracy of these tests should be verified and improved if possible.
View Article and Find Full Text PDFPhys Med Biol
January 2025
Electrical and Computer Engineering, University of Massachusetts Lowell, Ball Hall, 1 University Ave, Lowell, Massachusetts, 01854, UNITED STATES.
Objective: X-ray photon-counting detectors (PCDs) have recently gained popularity due to their capabilities in energy discrimination power, noise suppression, and resolution refinement. The latest extremity photon-counting computed tomography (PCCT) scanner leverages these advantages for tissue characterization, material decomposition, beam hardening correction, and metal artifact reduction. However, technical challenges such as charge splitting and pulse pileup can distort the energy spectrum and compromise image quality.
View Article and Find Full Text PDFmBio
January 2025
Institute for General Microbiology, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
Structural maintenance of chromosomes (SMC) are ubiquitously distributed proteins involved in chromosome organization. Deletion of causes severe growth phenotypes in many organisms. Surprisingly, can be deleted in , a member of the phylum, without any apparent growth phenotype.
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