Ann Clin Microbiol Antimicrob
Tibbi Biyoloji AD, KOU Tip Fakultesi, Izmit, Turkey.
Published: January 2010
Background: We studied the beta-lactamases of an E. aerogenes isolate recovered from the blood of a two-year-old patient. The isolate demonstrated a disk-diffusion phenotype typical for an AmpC-ESBL co-producer.
Methods: Microbiology studies were performed according to standard protocols. The resistance gene was identified by transconjugation and cloning experiments.
Results: By transconjugation only a narrow spectrum beta-lactamase (TEM-1) encoded on a small plasmid was transmitted. The ESBL was cloned and expressed in an E. coli host. Sequence analysis of the recombinant plasmid revealed blaSHV-12 associated to the insertion sequence, IS26.
Conclusion: This is the first study demonstrated the occurrence of SHV-12 in Nigeria.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817643 | PMC |
http://dx.doi.org/10.1186/1476-0711-9-1 | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!
© LitMetric 2025. All rights reserved.