Objective: To investigate the apoptosis induced by Pteris semipnnata L 5F (PsL5F) in human anaplastic thyroid carcinoma FRO cells and its molecular mechanism.
Methods: Human anaplastic thyroid carcinoma FRO cells were treated with PsL5F, and the growth inhibition rate was evaluated by MTT assay. The cell apoptosis rate was assessed by Annexin V-FITC fluorescence staining and flow cytometry. Intracellular reactive oxygen species (ROS) levels were analyzed by CM-H2DCFDA fluorescence staining and flow cytometry. Mitochondrial membrane potential (MMP) was measured by DiOD6 (3) fluorescence staining and flow cytometry. The levels of Bax, Cyto C, AIF and cleaved PARP were analyzed by Western blotting. The activity of caspase-3 was assayed by caspase-3 colorimetric assay kit.
Results: PsL5F has significant growth inhibitory action on FRO cells in dose and time dependent manners. Under the treatment of 100 mg/L of PsL5F, the percentage of apoptotic cells with phosphatidylserine (PS) externalization was gradually increased in time dependent manner. The rise of ROS level in FRO cells was observed as early as 1h after treated with PsL5F. The elevation of intracellular ROS levels and cell apoptosis could be inhibited by glutathione (GSH), a scavenger of ROS. The MMP in FRO cells was gradually reduced by PsL5F, and the reduction of MMP can be inhibited by GSH. Meanwhile, the levels of Bax in fraction of mitochondrial membrane, Cyto C and AIF in fraction of cytosol were gradually increased. PsL5F can cause the increase of caspase-3 activity and cleavage of PARP, a substrate of caspase-3.
Conclusion: PsL5F can inhibit growth of human anaplastic thyroid carcinoma FRO cells through inducing apoptosis. The rise of ROS levels in FRO cells plays important role as a secondary messenger in apoptosis induced by PsL5F. Mitochondrium is an important target of PsL5F. Cell apoptosis induced by PsL5F in FRO cells was carried out through translocation of Bax to mitochondrial membrane, reduction of MMP, release of Cyto C and AIF from mitochondria to cytosol, and activation of caspases cascade reaction.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Anaplastic thyroid carcinoma: vimentin segregates at the invasive front of tumors in a murine xenograft model.
Histochem Cell Biol
November 2024
Department of Experimental Medicine, University of Salento, 73100, Lecce, Italy.
Anaplastic thyroid carcinoma (ATC) ranks among the most lethal human cancers. Increased migratory and invasive capabilities are critical in malignancy and are often secondary to epithelial-mesenchymal transition (EMT). However, it is not clear whether the invasive behavior of ATC is associated with the presence of EMT.
View Article and Find Full Text PDFDevelopment of scaffold-free tissue-engineered constructs derived from mesenchymal stem cells with serum-free media for cartilage repair and long-term preservation.
Cytotechnology
October 2024
Department of Orthopaedics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871 Japan.
Unlabelled: Synovial mesenchymal stem cells (sMSCs) have great potential for cartilage repair, but their therapeutic design to avoid adverse effects associated with unknown factors remains a challenge. In addition, because long-term preservation is indispensable to maintain high quality levels until implantation, it is necessary to reduce their fluctuations. This study aimed to investigate the properties and feasibility of novel scaffold-free tissue-engineered constructs using serum-free media and to develop long-term preservation methods.
View Article and Find Full Text PDFTIM3 activates the ERK1/2 pathway to promote invasion and migration of thyroid tumors.
PLoS One
April 2024
The Third Department of External Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Background: This study aims to study the possible action mechanism of T-cell immunoglobulin and mucin domain 3 (TIM3) on the migratory and invasive abilities of thyroid carcinoma (TC) cells.
Methods: GSE104005 and GSE138198 datasets were downloaded from the GEO database for identifying differentially expressed genes (DEGs). Functional enrichment analysis and protein-protein interaction (PPI) analysis were performed on the common DEGs in GSE104005 and GSE138198 datasets.
Systematic immune cell dysregulation and molecular subtypes revealed by single-cell RNA-seq of subjects with type 1 diabetes.
Genome Med
March 2024
Laboratory of Systems Biology and Data Analytics, Genome Institute of Singapore (GIS), A*STAR (Agency for Science, Technology and Research), Singapore, 138672, Singapore.
Article Synopsis
- Type 1 diabetes (T1DM) is an autoimmune disease that destroys insulin-producing cells in the pancreas, and this study aimed to evaluate immune cell changes at the single-cell level for the first time.
- Researchers analyzed immune cells from 46 T1DM patients and 31 controls, finding significant gene expression alterations in immune cells that were greater than those observed in another autoimmune disease, systemic lupus erythematosus (SLE).
- The study developed a new metric, the T1DM metagene z-score (TMZ score), which could categorize patients, correlate with existing immune markers, and help inform treatment responses, highlighting the major immune shifts present in T1DM.
Mechanisms of vemurafenib-induced anti-tumor effects in ATC FRO cells.
Heliyon
March 2024
Research Institute of Medicine and Pharmacy of Qiqihar Medical University, Heilongjiang, 16006, China.
Background: Anaplastic Thyroid Carcinoma (ATC) is a rare and deadly malignant tumor in humans. It is prone to developing resistance to radiotherapy and chemotherapy. Molecular targeted therapy offers a novel way to treat ATC.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!