Carbon source is the main factor influencing biological denitrification efficiency. In most cities of China, carbon content in sewage was observed to be low, herein carbon source supplement should be considered to provide electron donors needed in biological denitrification process. The influence of adding different carbon sources through aeration pipe of integrated vertical-flow constructed wetland (IVCW) on nitrogen removal had been studied. Carbon source supplement to the bottom of IVCW could improve microbe conditions and intensify nitrogen removalfunction of IVCW. The results showed that glucose as external carbon source was better than carboxymethyl cellulose (CMC) on denitrification. Nitrogen removal had significant difference between adding glucose and no carbon source in IVCW system (p < 0.05). By the experiments of adding different quantity of glucose, the dose of 1.5 g glucose under 60 L x d(-1) hydraulic load was the optimization for denitrification. C6H12O6:NO3(-) -N was 4.3 and far lower than that by adding in inflow. So carbon source supplement to the bottom of IVCW through aeration pipe could save carbon source supplement cost. Additionally, adding glucose for four hours before influent feeding could improve nitrogen removal.
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