An optimized method for DNA extraction from materials available for forensic medical examination is proposed based on partial combination of two procedures, phenol-chloroform extraction and sorption. Such a combination provided a basis for the development of a protocol making use of the advantages of both procedures and free of drawbacks of either of them. This relatively simple and rapid method uses no reagents having adverse effect on human organism. Also, it excludes the use of substances interfering with polymerase chain reaction (PCR) and thereby reduces the risk of DNA loss. This makes it suitable for the work with objects having very low DNA content. The DNA specimens obtained with the help of the proposed technique are fit for prolonged storage.
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J Allergy Clin Immunol
January 2025
Departments of Animal Science, Integrative Biology and Physiology, University of Minnesota,St. Paul, MN, 55108. Electronic address:
Background: Environmental allergens induce the release of danger signals from the airway epithelium that trigger type 2 immune responses and promote airway inflammation.
Objective: To investigate the role of allergen-stimulated P2Y receptor activation in regulating ATP, IL-33 and DNA release by human bronchial epithelial (hBE) cells and mouse airways.
Methods: hBE cells were exposed to Alternaria alternata extract and secretion of ATP, IL-33 and DNA were studied in vitro.
Viruses
January 2025
Section of Dermatology, Department of Medical and Surgical Sciences, University of Foggia, Viale Pinto 1, 71122 Foggia, Italy.
Pityriasis rosea (PR) is a self-limited exanthem associated with the endogenous systemic reactivation of human herpesvirus (HHV)-6 and HHV-7. The disease typically begins with a single erythematous patch on the trunk (herald patch), followed by a secondary eruption of smaller papulosquamous lesions. Rarely, the herald patch may be the only cutaneous manifestation of PR.
View Article and Find Full Text PDFViruses
January 2025
Laboratory of Gastroenteric Virus, Virology Section, Evandro Chagas Institute, Secretariat of Health Surveillance and Environment, Brazilian Ministry of Health, Ananindeua 67.030-000, Pará, Brazil.
Acute gastroenteritis (AG) is a major illness in early childhood. Recent studies suggest a potential association between human bocavirus (HBoV) and AG. HBoV, a non-enveloped virus with a single-strand DNA genome, belongs to the Parvoviridae family.
View Article and Find Full Text PDFViruses
December 2024
Department of Rehabilitation and Regenerative Medicine, College of Physicians and Surgeons, Columbia University, HHSC-1518, 701 W. 168th Street, New York, NY 10032, USA.
This study explores the effects of plant compounds on human papillomavirus (HPV)-induced W12 cervical precancer cells and bioelectric signaling. The aim is to identify effective phytochemicals, both individually and in combination, that can prevent and treat HPV infection and HPV associated cervical cancer. Phytochemicals were tested using growth inhibition, combination, gene expression, RT PCR, and molecular docking assays.
View Article and Find Full Text PDFPlants (Basel)
January 2025
Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Gral. Ramón Corona No 2514, Colonia Nuevo México, Zapopan 45121, Mexico.
In this work, extracts from the pulp, peel, and seed of were obtained via lyophilization and oven drying. Bromatological analyses were performed to investigate variabilities in the nutritional content of fruits after nine post-harvest days. The phytochemical content of fruits was assessed by gas chromatography flame ionization detector (GC-FID), and their biological performance was studied using antibacterial and antioxidant assays (DPPH and ABTS) and toxicity models.
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