Type II and III Taste Bud Cells Preferentially Expressed Kainate Glutamate Receptors in Rats.

Korean J Physiol Pharmacol

Department of Physiology and Neuroscience, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, Korea.

Published: December 2009

Glutamate-induced cobalt uptake reveals that non-NMDA glutamate receptors (GluRs) are present in rat taste bud cells. Previous studies involving glutamate induced cobalt staining suggest this uptake mainly occurs via kainate type GluRs. It is not known which of the 4 types of taste bud cells express subunits of kainate GluR. Circumvallate and foliate papillae of Sprague-Dawley rats (45~60 days old) were used to search for the mRNAs of subunits of non-NMDA GluRs using RT-PCR with specific primers for GluR1-7, KA1 and KA2. We also performed RT-PCR for GluR5, KA1, PLCbeta2, and NCAM/SNAP 25 in isolated single cells from taste buds. Taste epithelium, including circumvallate or foliate papilla, express mRNAs of GluR5 and KA1. However, non-taste tongue epithelium expresses no subunits of non-NMDA GluRs. Isolated single cell RT-PCR reveals that the mRNAs of GluR5 and KA1 are preferentially expressed in Type II and Type III cells over Type I cells.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2802306PMC
http://dx.doi.org/10.4196/kjpp.2009.13.6.455DOI Listing

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