Background: Microscopy is usually used to obtain manual total and differential cell counts in equine synovial fluid. A faster, more precise method is desirable.
Objectives: The objectives were to compare an automated impedance method with a manual method for obtaining total and differential cell counts in equine synovial fluid and to evaluate the effect of pretreatment with hyaluronidase on automated results.
Methods: Synovial fluid samples (n=48) were collected into EDTA and analyzed within 48 hours. Automated total and differential cell counts were evaluated using a Medonic CA620-VET hematology analyzer before and after pretreatment for 5-30 minutes with hyaluronidase (final concentration 0.01 mg/mL). A hemacytometer count and microscopic evaluation of a direct smear were used as the reference method. Intra-assay coefficients of variation (CV) were determined.
Results: Thirty-one of 46 untreated samples and 0/46 hyaluronidase-treated samples were error-flagged by the analyzer. Correlation between automated (ANCC) and manual (MNCC) nucleated cell counts in untreated samples (n=15; R(2)=0.93) and pretreated samples (n=46; R(2)=0.94) was high, and pseudomedian difference was low. Intra-assay CVs for samples with medium and high cellularity were significantly lower for ANCC (1.5-2.7%) compared with MNCC (6.1-15.7%) (P<.01). Valid automated differential cell counts were not obtained.
Conclusions: Automated total cell counts obtained on the Medonic analyzer correlate well with manual counts in equine synovial fluid; however, pretreatment with hyaluronidase is required to minimize error flags. Automated differential counts are not accurate for synovial fluid.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1939-165X.2009.00203.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Novel Polarized Light Microscope for the Examination of Birefringent Crystals in Synovial Fluid.
Gout Urate Cryst Depos Dis
December 2024
Electrical and Computer Engineering Department, University of California, Los Angeles, CA 90095, USA.
Background: The gold standard for crystal arthritis diagnosis relies on the identification of either monosodium urate (MSU) or calcium pyrophosphate (CPP) crystals in synovial fluid. With the goal of enhanced crystal detection, we adapted a standard compensated polarized light microscope (CPLM) with a polarized digital camera and multi-focal depth imaging capabilities to create digital images from synovial fluid mounted on microscope slides. Using this single-shot computational polarized light microscopy (SCPLM) method, we compared rates of crystal detection and raters' preference for image.
View Article and Find Full Text PDFExploring the prevalence of gout among underrepresented low socioeconomic status type 2 diabetes populations.
Diabetol Metab Syndr
January 2025
Philadelphia College of Osteopathic Medicine, Philadelphia, USA.
Background: Underserved and underrepresented populations often lack access to affordable, quality healthcare, educational resources, and nutritious foods, all of which contribute to increased risk of Type 2 Diabetes and gout. Type 2 Diabetes is a condition characterized by the denaturation of the insulin receptors, due to chronically high blood glucose levels, leading to impaired regulation of blood sugar. Gout is a chronic inflammatory disease affecting joints in the lower limbs, marked by elevated serum urate levels and the accumulation of uric acid crystals in synovial fluid, causing painful flare-ups that significantly impact quality of life.
View Article and Find Full Text PDFExercise induces dynamic changes in intra-articular metabolism and inflammation associated with remodeling of the infrapatellar fat pad in mice.
Sci Rep
January 2025
Department of Mechanical & Industrial Engineering, Montana State University, Bozeman, MT, 59717, USA.
We hypothesized that daily exercise promotes joint health by upregulating anti-inflammatory mediators via adaptive molecular and metabolic changes in the infrapatellar fat pad (IFP). We tested this hypothesis by conducting time-resolved analyses between 1 and 14 days of voluntary wheel running exercise in C57BL/6J mice. IFP structure and cellularity were evaluated by histomorphology, picrosirius red collagen staining, and flow cytometry analysis of stromal vascular fraction cells.
View Article and Find Full Text PDFSafety of vancomycin-loaded cement spacers for treating gram-positive periprosthetic joint infections in two-stage resection arthroplasty among patients with renal insufficiency.
BMC Musculoskelet Disord
January 2025
Institute of Statistics, National Yang Ming Chiao Tung University, 1001 University Road, Hsinchu City, 300, Taiwan.
Background: The standard treatment for periprosthetic joint infections (PJI) typically involves a two-stage resection arthroplasty using antibiotic-loaded bone cement (ALBC) spacers. This study hypothesizes that there is no significant correlation between antibiotic levels in blood and synovial fluid and the patient's kidney function, and that the success rates of staged resection arthroplasty are comparable between groups, specifically targeting gram-positive bacterial infections.
Methods: This retrospective review included patients treated from 2017 to 2022 with two-stage arthroplasty using vancomycin-loaded ALBC spacers, selectively targeting gram-positive infections.
Injectable vancomycin loaded hyaluronic acid-chitosan hydrogel for the treatment of Staphylococcus aureus septic arthritis.
Carbohydr Res
January 2025
School of Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, 682041, India. Electronic address:
Staphylococcus aureus (S. aureus) is a Gram positive opportunistic pathogen and a major cause for bacterial septic arthritis. Vancomycin is the preferred antibiotic for the treatment of methicillin resistance S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!