Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions.

Appl Environ Microbiol

Graduate School of Science and Engineering, Kagoshima University, Korimoto 1-21-35, Kagoshima 890-0065, Japan.

Published: March 2010

Frankia is an actinobacterium that fixes nitrogen under both symbiotic and free-living conditions. We identified genes upregulated in free-living nitrogen-fixing cells by using suppression subtractive hybridization. They included genes with predicted functions related to nitrogen fixation, as well as with unknown function. Their upregulation was a novel finding in Frankia.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832394PMC
http://dx.doi.org/10.1128/AEM.01813-09DOI Listing

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