pilF Polymorphism-based PCR to distinguish vibrio vulnificus strains potentially dangerous to public health.

Appl Environ Microbiol

Departamento de Microbiología, Facultad de Biología, Universidad de Valencia, Valencia 46100, Spain.

Published: March 2010

Vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. In this report, we describe a PCR-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. Discrimination is based on the amplification of a variable region located within the gene pilF, which seems to be associated with potential human pathogenicity, regardless of the biotype of the strain.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2832365PMC
http://dx.doi.org/10.1128/AEM.01042-09DOI Listing

Publication Analysis

Top Keywords

vibrio vulnificus
8
dangerous public
8
public health
8
pilf polymorphism-based
4
polymorphism-based pcr
4
pcr distinguish
4
distinguish vibrio
4
vulnificus strains
4
strains dangerous
4
health vibrio
4

Similar Publications

Genome-wide phenotypic profiling of transcription factors and identification of novel targets to control the virulence of Vibrio vulnificus.

Nucleic Acids Res

December 2024

National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.

For successful infection, the life-threatening pathogen Vibrio vulnificus elaborately regulates the expression of survival and virulence genes using various transcription factors (TFs). In this study, a library of the V. vulnificus mutants carrying specific signature tags in 285 TF genes was constructed and subjected to 16 phenotypic analyses.

View Article and Find Full Text PDF

Galectins exhibit a variety of biological functions through interactions with their ligands, including galactose and its derivatives. Tandem-repeat galectins, such as Galectin-8, can act as pattern recognition receptors to aggregate and neutralize bacterial pathogens. In this study, Galectin-8 was identified in Trachinotus ovatus (golden pompano).

View Article and Find Full Text PDF

Climate change is having increasingly profound effects on human health, notably those associated with the occurrence, distribution, and transmission of infectious diseases. The number of disparate ecological parameters and pathogens affected by climate change are vast and expansive. Disentangling the complex relationship between these variables is critical for the development of effective countermeasures against its effects.

View Article and Find Full Text PDF

Background: Recent advancements in artificial intelligence have led to increased adoption of machine learning in disease identification, particularly for challenging diagnoses like necrotizing fasciitis and infections. This shift is driven by the technology's efficiency, objectivity, and accuracy, offering potential solutions to longstanding diagnostic hurdles in clinical practice.

Methods: This investigation incorporated 180 inpatients suffering from soft tissue infections.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!