Background And Objective: Multinodular hepatocellular carcinoma(HCC) might originate from multicentric occurrence (MO) or intrahepatic metastasis(IM). This study was to find out proteins which play important roles in clonal origin of multinodular hepatocellular carcinoma bt screening the differentially expressed proteins between the MO and IM tissues using comparative proteomic analysis.

Methods: Total protein extracted was separated by two-dimensional gel electrophoresis. Comparative analyses of the 2-DE protein patterns between the two groups were carried out using computerized imaging techniques. Proteins exhibiting significant alternations were subsequently isolated and identified by mass spectrometry.

Results: A total 1025+/-52 and 900+/-98 spots were detected in the protein profile in IM and MO, respectively. Twenty-five protein spots were statistically different at expression levels between the two groups. Twenty of them were identified by MALDI-TOF-MS and bioinformatics.

Conclusions: The protein profile of MO HCC tissues is different from that in IM HCC tissues. The twenty differentially expressed proteins might play a key role in the carcinogenesis and progression of multinodular HCC. These newly identified proteins might be potential and valuable biomarkers for identifying the multinodular HCC of clonal origin.

Download full-text PDF

Source
http://dx.doi.org/10.5732/cjc.009.10336DOI Listing

Publication Analysis

Top Keywords

multinodular hepatocellular
12
multicentric occurrence
8
occurrence intrahepatic
8
proteins play
8
clonal origin
8
differentially expressed
8
expressed proteins
8
protein profile
8
hcc tissues
8
multinodular hcc
8

Similar Publications

Background: In hepatocellular carcinoma (HCC), postoperative recurrence remains high. This study aimed to evaluate the recurrence patterns and prognosis of HCC after curative hepatectomy.

Methods: Among 352 patients with primary HCC who underwent initial hepatectomy between January 2002 and December 2022, 151 with recurrence were assessed for the relationship between recurrence pattern and prognosis.

View Article and Find Full Text PDF
Article Synopsis
  • * A case study details a 14-year-old boy with HBV who developed multinational HCC, presenting symptoms like abdominal distension and jaundice and confirmed through CECT and histopathology.
  • * The child's presentation mirrored that of adults, with risk factors including viral hepatitis and male gender; notably, he had no previous signs of liver issues before being diagnosed.
View Article and Find Full Text PDF
Article Synopsis
  • The study looked at using CT scans and medical information to predict a specific tumor pattern in liver cancer called VETC and how it affects patient recovery.
  • Researchers analyzed data from 243 liver cancer patients who had surgery after getting CT scans.
  • They found some important signs, like high bilirubin levels and certain tumor features, that could indicate a higher chance of the cancer returning after surgery.
View Article and Find Full Text PDF

Introduction: The phase III REFLECT trial demonstrated that lenvatinib was superior to sorafenib in terms of progression-free survival (PFS), time to progression, and objective response rate (ORR) for patients with unresectable hepatocellular carcinoma (HCC). This study assessed the efficacy and safety of preoperative lenvatinib therapy for patients with oncologically or technically unresectable HCC.

Methods: In this multicenter single-arm phase II trial, patients with advanced HCC and factors suggestive of a poor prognosis (macroscopic vascular invasion, extrahepatic metastasis, or multinodular tumors) were enrolled.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!