AI Article Synopsis
- Sperm disomy levels are linked to infertility and raise concerns about the risk of genetic issues in offspring from infertile males undergoing ICSI.
- The main challenge to implementing sperm disomy screening in clinics is the time needed for accurate analysis by operators.
- A study comparing manual scoring and automated counting found a significant correlation for sperm disomy scoring but not for diploid sperm, indicating potential errors in scoring methods.
Article Abstract
It is now well known that levels of sperm disomy correlate to levels of infertility (as well as other factors). The risk of perpetuating aneuploidy to the offspring of infertile males undergoing intracytoplasmic sperm injection (ICSI) has become a hotly debated issue in assisted reproduction; however, there remain barriers to the practical implementation of offering sperm disomy screening in a clinical setting. The major barrier is the operator time taken to analyze a statistically meaningful (sufficient) number of cells. The introduction of automated 'spot counting' software-hardware combinations presents a potential solution to this problem. In this preliminary validation study, we analyzed 10 patients, both manually and using a commercially available spot counter. Results show a statistically significant correlation between both approaches for scoring of sperm disomy, but no correlation is found when scoring for diploid sperm. The most likely explanation for the latter is an apparent overscoring of two closely associated sperm heads as a single diploid cell. These results, and similar further studies that will ensue, help to inform cost-benefit analyses that individual clinics need to carry out in order to decide whether to adopt sperm aneuploidy screening as a routine tool for the assessment of sperm from men requiring ICSI treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3739098 | PMC |
| http://dx.doi.org/10.1038/aja.2009.85 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rituximab alleviates increased disomic sperm in DBA/1J mouse models of rheumatoid arthritis via restoration of redox imbalance.
J Biochem Mol Toxicol
December 2023
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Compared to the general population, patients with arthritis have a higher risk of fertility abnormalities, which have deleterious effects on both reproductive function and pregnancy outcomes, especially in patients wishing to conceive. These may be due to the disease itself or those of drug therapies. Despite the increasing use of rituximab in arthritis, limited data are available on its potential to induce aneuploidy in germ cells.
View Article and Find Full Text PDFPolymorphic Rearrangements of Human Chromosome 9 and Male Infertility: New Evidence and Impact on Spermatogenesis.
Biomolecules
April 2023
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
Chromosomal polymorphisms are structural variations in chromosomes that define the genomic variance of a species. These alterations are recurrent in the general population, and some of them appear to be more recurrent in the infertile population. Human chromosome 9 is highly heteromorphic, and how its rearrangement affects male fertility remains to be fully investigated.
View Article and Find Full Text PDFRisk assessment of assisted reproductive technology and parental age at childbirth for the development of uniparental disomy-mediated imprinting disorders caused by aneuploid gametes.
Clin Epigenetics
May 2023
Department of Molecular Endocrinology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-Ku, Tokyo, 157-8535, Japan.
Article Synopsis
- A study indicated that assisted reproductive technology (ART) might be a risk factor for epimutation-mediated imprinting disorders in mothers aged 30 and older, but its effect on uniparental disomy-mediated imprinting disorders (UPD-IDs) remains uncertain.
- Researchers assessed 130 patients with aneuploid UPD-IDs and compared ART-conceived birth rates and maternal ages with those in the general population and patients with epi-IDs, finding no significant differences, although UPD-IDs patients tended to be older.
- The analysis revealed that nearly all ART-conceived births occurred in patients with aneuploid oocyte-derived UPD-IDs (oUPD-IDs), who also had significantly older
A novel loss-of-function variant in PNLDC1 inducing oligo-astheno-teratozoospermia and male infertility.
Asian J Androl
September 2023
Reproductive Medicine Center, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China.
Male infertility is a major reproductive disorder, which is clinically characterized by highly heterogeneous phenotypes of abnormal sperm count or quality. To date, five male patients with biallelic loss-of-function (LOF) variants of PARN-like ribonuclease domain-containing exonuclease 1 ( PNLDC1 ) have been reported to experience infertility with nonobstructive azoospermia. The aim of this study was to identify the genetic cause of male infertility with oligo-astheno-teratozoospermia (OAT) in a patient from a Chinese Han family.
View Article and Find Full Text PDFGenetic analysis of recurrent parthenogenesis: A case report and literature review.
Exp Ther Med
August 2022
The Center for Reproductive Medicine and Infertility, The Fourth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
The present study reported a case of bilateral salpingectomy for an ectopic pregnancy with recurrent parthenogenesis over two fertilization (IVF) cycles. The first IVF cycle resulted in short-time fertilization. Two cleaved embryos were present after removing the cumulus cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!