AI Article Synopsis
- Research shows that changes in the shape of dendritic spines, important for synaptic plasticity, are influenced by the actin cytoskeleton and regulated by Rho GTPases, specifically RhoA, which is found in dendrites of neurons.
- Scientists developed an improved method to isolate synaptoneurosomes from mouse brain tissue, resulting in samples that are richer in synaptic proteins and contain a lot of dendritic mRNAs, enhancing study accuracy.
- The study found that RhoA mRNA is localized in dendrites and its translation increases when stimulated by BDNF, suggesting RhoA may play a role in controlling spine morphology through its local translation.
Article Abstract
Changes in dendritic spine morphology, a hallmark of synaptic plasticity, involve remodeling of the actin cytoskeleton, a process that is regulated by Rho GTPases. RhoA, a member of this GTPase family, segregates to dendrites in differentiated neurons. Given the emerging role of dendritic mRNA local translation in synaptic plasticity, we have assessed the possible localization and translation of RhoA mRNA at dendrites. At this end, we have developed and describe here in detail an improved method for isolating hippocampal and neocortical mouse synaptoneurosomes. This synaptoneurosomal preparation is much more enriched in synaptic proteins than those obtained in former methods, exhibits bona fide electron microscopy pre- and postsynaptic morphologies, contains abundant dendritic mRNAs, and is competent for activity-regulated protein synthesis. Using this preparation, we have found that RhoA mRNA is dendritically localized and its local translation is enhanced by BDNF stimulation. These findings suggest that some of the known functions of RhoA on spine morphology may be mediated by regulating its local translation.
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| http://dx.doi.org/10.1016/j.mcn.2009.12.004 | DOI Listing |
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