Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge.

J Microbiol Methods

USDA-ARS, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA.

Published: February 2010

To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may be appropriate for some experimental systems.

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http://dx.doi.org/10.1016/j.mimet.2009.12.007DOI Listing

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