Genomic DNA from the silk moth Antheraea pernyi bearing the gene of a pheromone binding protein has been isolated from a partial genomic library using specific cDNA probes. The DNA spans 3.5 kilobases, contains three exons and two intervening sequences that interrupt the protein coding region of the gene. A DNA fragment of a second gene was isolated and the complete primary structure of a corresponding cDNA clone was unravelled. The expression of two different genes, giving rise to different pheromone binding proteins, implies a more specific function of these proteins than was hitherto assumed.
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http://dx.doi.org/10.1016/0167-4781(91)90064-s | DOI Listing |
Insects
November 2024
Shaanxi Province Key Laboratory of Jujube, College of Life Science, Yan'an University, Yan'an 716000, China.
The plum fruit moth (PFM), , and the oriental fruit moth (OFM), , are closely related fruit moth species that severely damage fruit trees in Rosaceae. Both species share common primary sex pheromone components 8-12:Ac and 8-12:Ac. The secondary sex pheromone components of PFMs consist of 8-12:OH, 8-14:Ac, and 10-14:Ac, while those of OFMs include 8-12:OH and 12:OH.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:
Odorant binding proteins (OBPs) play key roles in the insect olfactory system by assisting the neuronal response to hydrophobic odor molecules, understanding their interaction with ligands will facilitate the virtual screening of behaviorally active compounds in insects. Here, we successfully cloned and confirmed CmedOBP13, an antennae-biased OBP from the rice leaffolder Cnaphalocrocis medinalis, as a secreted protein. Recombinant CmedOBP13 was obtained using the Escherichia coli system, and its binding affinities to 35 volatile compounds emitted by rice plants and three sex pheromone components from female moths were assessed by a competitive binding assay.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
Winged aphids develop more sensitive olfaction than the wingless phenotype to identify potential habitat from afar. Two types of olfactory sensilla, primary rhinarium (PRh) and secondary rhinarium (SRh) are responsible for aphid olfactory perception, of which, SRh is involved in the perception of both E-β-farnesene (EBF) and plant volatiles. Odorant binding proteins (OBPs) play a vital role in the response of insect olfactory nerves located in the rhinarium to external odor stimuli.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
State Key Laboratory of Wheat Improvement, Shandong Agricultural University, No. 61, Daizong Road, Taian 271018, China.
Moths use pheromones to ensure intraspecific communication. Nevertheless, few studies are focused on both intra- and intersexual communication based on pheromone recognition. Pheromone-binding proteins (PBPs) are generally believed pivotal for male moths in recognizing female pheromones.
View Article and Find Full Text PDFPestic Biochem Physiol
December 2024
Zhongkai University of Agriculture and Engineering, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, PR China. Electronic address:
Pheromone-binding proteins (PBPs) are mainly responsible for binding and transporting hydrophobic pheromone molecules across the aqueous sensilla lymph to the receptor proteins. The preference of each PBP is believed to be different for each pheromone component within a single species. Significantly higher expression level of PBP1 and PBP2 in the male antennae of Spodoptera frugiperda suggesting that SfruPBP1 and SfruPBP2 might play important roles in pheromone perception.
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