MALDI-TOF mass spectrometry approaches to the characterisation of insect neuropeptides.

Methods Mol Biol

The Food and Environment Research Agency, Sand Hutton, York, UK.

Published: February 2010

AI Article Synopsis

  • - Identifying insect neuropeptides is tough due to their diversity and the insects' small size, making peptide sequencing challenging.
  • - New mass spectrometry techniques, like MALDI-TOF and ESI-Q-TOF, combined with insect genome data, have made it easier to analyze neuropeptides from just a few cells instead of needing many insects.
  • - Once neuropeptides are identified, researchers can better understand their physiological roles and functions.

Article Abstract

The diversity of insect neuropeptides coupled with the limitations from the small size of the insects themselves combine to make positive identification through peptide sequencing a highly challenging task. The advent of the "soft-ionisation" techniques of MALDI-TOF and electrospray (ESI)-Q-TOF mass spectrometry, coupled with the additional information from insect genome projects have revolutionised the characterisation of insect neuropeptides, such that sequences can now be obtained from just a few cells, where before thousands of insects had to be laboriously dissected, extracted and purified. Some of the procedures that are now used to identify these peptides are described here. Once the neuropeptides have been identified, it then becomes possible to use this knowledge to define physiological functionality.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-60761-535-4_8DOI Listing

Publication Analysis

Top Keywords

insect neuropeptides
12
mass spectrometry
8
characterisation insect
8
maldi-tof mass
4
spectrometry approaches
4
approaches characterisation
4
insect
4
neuropeptides
4
neuropeptides diversity
4
diversity insect
4

Similar Publications

Alcohol consumption and addiction remain global health concerns, with significant loss of productivity, morbidity, and mortality. , a widely used model organism, offers valuable insights into the genetic and neuronal mechanisms underlying ethanol-induced behaviors (EIBs) such as sedation, recovery, and tolerance. This narrative review focuses on studies in the model system suggesting an association between circadian rhythm genes as modulators of ethanol tolerance.

View Article and Find Full Text PDF

PKC phospho-activated PFK1 is required for PBAN regulated sex pheromone biosynthesis in Helicoverpa armigera.

J Insect Physiol

December 2024

State Key Laboratory of Wheat and Maize Crop Science/Henan International Laboratory for Green Pest Control/College of Plant Protection, Henan Agricultural University, Zhengzhou, China. Electronic address:

The enzyme 6-phosphofructokinase-1 (PFK1) acts as the primary rate-limiting enzyme in glycolysis, catalyzing the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate. This glycolytic process provides essential substrates for the synthesis of sex pheromones. However, the specific function of PFK1 in sex pheromone biosynthesis remains unidentified.

View Article and Find Full Text PDF

AKH/AKHR signalling system induced antioxidant response mediated by entomopathogenic fungi in Nilaparvata lugens (Stål).

Pestic Biochem Physiol

December 2024

National Biopesticide Engineering Research Centre, Hubei Biopesticide Engineering Research Centre, Hubei Academy of Agricultural Science, Wuhan 430064, PR China. Electronic address:

The brown planthopper Nilaparvata lugens is one of the most economically important rice crop pests in Asia. Entomopathogenic fungi (EPF) have been developed as a biological control of N. lugens.

View Article and Find Full Text PDF

A brief history of insect neuropeptide and peptide hormone research.

Cell Tissue Res

December 2024

Department of Zoology, Stockholm University, S-10691, Stockholm, Sweden.

This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing of proctolin in 1975. The first 25 years, before the sequencing of the Drosophila genome, were characterized by efforts to identify novel NPHs by biochemical means, mapping of their distribution in neurons, neurosecretory cells, and endocrine cells of the intestine. Functional studies of NPHs were predominantly dealing with hormonal aspects of peptides and many employed ex vivo assays.

View Article and Find Full Text PDF

Diuretic hormones (DHs) activate corresponding G protein-coupled receptors (GPCRs), mediating the water and ion homeostasis in arthropods. There are two different DHs known to be expressed in insects, calcitonin (CT)-like DH31 and corticotropin-releasing factor (CRF)-like DH44. In this study, we identified and characterized a DH44 and five GPCR variants, DH44-R1 and DH44-R2a/b/c/d, in Drosophila suzukii (spotted-wing drosophila), causing detrimental damage to fresh and soft-skinned fruits.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: