AI Article Synopsis
- The study introduces a new fluorescence assay designed to detect small amounts of ocratoxin A and neomycin in food products.
- The assay operates by measuring changes in fluorescein-labeled analytes with and without the presence of specific anti-analyte antibodies.
- Results showed that using high-avidity IgG antibodies in this innovative immunoassay allowed for extremely low detection limits of 0.0078 ng for ocratoxin A and 0.0156 ng for neomycin.
Article Abstract
In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.
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| http://dx.doi.org/10.2174/092986609789839359 | DOI Listing |
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