The water diffusional permeability of human red blood cells following exposure to various sulfhydryl group (SH) reagents have been studied using a nuclear magnetic resonance technique. Exposure of red blood cells up to 12 mM N-ethylmaleimide (NEM) or 10 mM 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNE) alone does not affect water diffusion. In contrast, when DTNB treatment follows a preincubation of the cells with NEM, a small (18% at 37 degrees C) but significant inhibition of water permeability occurs. The NEM and DTNB treatment of the cells caused no change of the cell shape and volume or of the cell water volume. Consequently, the inhibition observed after NEM and DTNB treatment has a real significance.
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Article Synopsis
- Sepsis and septic shock are critical health issues linked to high mortality rates, with the inflammatory response playing a major role in organ dysfunction, particularly affecting the cardiovascular system through severe hypotension.* -
- Nitric oxide (NO) is a pivotal factor in both inflammation and cardiovascular issues during sepsis, influencing proteins through post-translational modifications, and DTNB is utilized to study these interactions by targeting reactive thiol groups in proteins.* -
- In experiments with sepsis-induced mice, DTNB treatment reduced lung vascular leakage, lowered nitrite/nitrate levels, and diminished inflammatory markers like IL-1ß, suggesting its potential benefits in managing sepsis-induced inflammation.*
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