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Anal Chem
November 2024
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
The colorimetric point-of-care test (POCT) offers a rapid and efficient method for detecting specific targets in real samples. However, traditional colorimetric methods often rely on complex signal amplification techniques or electronic devices to enhance detection sensitivity, which can inadvertently increase both cost and time, thus contradicting the fundamental goals of visual detection methods. Here, we presented a distance-based fluorescent immunosensor that utilized a gas-producing nanozyme for continuous gas production reaction as a signal.
View Article and Find Full Text PDFEnviron Sci Technol
October 2024
College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
Granule-based anaerobic ammonium oxidation (Anammox) is a promising biotechnology for wastewater treatments with extraordinary performance in nitrogen removal. However, traditional analytical methods often delivered an average activity of a bulk sample consisting of millions and even billions of Anammox granules with distinct sizes and components. Here, we developed a novel technique to monitor the biochemical activity of individual Anammox granules in real-time by recording the production rate of nitrogen gas with a microbarometer in a sealed chamber containing only one granule.
View Article and Find Full Text PDFFood Microbiol
December 2024
Sichuan Institute of Atomic Energy, Chengdu, 610101, Sichuan, PR China; Irradiation Preservation Key Laboratory of Sichuan Province, Chengdu, 610101, Sichuan, PR China. Electronic address:
The spoilage of irradiated ready-to-eat chicken feet (RTECF) seriously affects the food's quality, resulting in package swelling and off-flavors, both of which are highly undesirable to stakeholders and consumers. To investigate the spoilage characteristics of irradiated RTECF and the microorganisms responsible for the spoilage and swelling, the changes in physicochemical properties, microbial community, and volatile organic compounds (VOCs) between normal and spoiled RTECF were evaluated. Compared with normal samples, the spoiled RTECF showed a higher pH value and total volatile basic nitrogen (TVB-N) value, lower color value, and texture features (P < 0.
View Article and Find Full Text PDFHeliyon
August 2024
Faculty of Engineering Management, NUST, Islamabad, Pakistan.
Pakistan has limited natural gas reserves, and most are found onshore. This article reports on the problems of an onshore gas gathering network (GGN) analysed through steady-state simulation modelling using PIPESIM software. The research methodology incorporates a comprehensive steady-state hydraulic analysis considering fluid flowing velocity limitations, liquid holdup and slugging along with other issues faced by gas gathering networks.
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