In an objective to find out an effective, nontoxic dendritic cell (DC) maturating agent for human use, CD14(+) monocytes were differentiated with GMCSF/IL-4 and matured with neem leaf glycoprotein (NLGP). NLGP matured DCs (NLGP-DCs) show upregulated expression of CD83, CD80, CD86, CD40 and MHCs, in a comparable extent of control, LPS. NLGP-DCs secrete high amount of IL-12p70 with low IL-10. NLGP upregulates the expression of crucial transcription factor, ikaros, indicating maturation towards DC1 phenotype. Increased expression of CD28 and CD40L on T cells following co-culture with NLGP-DCs was noticed to promote DC-T interactions. As a result, T cells secrete high amount of IFN gamma with low IL-4 and generates anti-tumor type 1 immune microenvironment. Such NLGP-DCs present carcinoembryonic antigen (CEA) effectively to T cells to increase T cell mediated cytotoxicity of CEA(+) tumor cells in vitro and in vivo. With emergence of the NLGP as a promising DC maturating agent, NLGP-DCs can be used as a candidate vaccine tool for antigen specific cancer immunotherapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.vaccine.2009.11.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
"Comparative evaluation of cytotoxicity of three herbal endodontic irrigants at three intervals of time" - An study.
J Conserv Dent Endod
November 2024
Department of Conservative Dentistry and Endodontics, Krishnadevaraya College of Dental Sciences and Hospital, Bengaluru, Karnataka, India.
Aim: The aim of the study was to evaluate and compare the cytotoxicity of 25% of neem leaf extract (), 20% of guava leaf extracts (), and 20% of cinnamon extract () irrigants at three intervals of time.
Methodology: Four groups were formed ( = 15), Group 1 (control group) - normal saline solution, Group 2 - 25% of neem extract, Group 3 - 20% of guava extract, and Group 4 - 20% of cinnamon extract. Each group was further divided into three subgroups based on intervals ( = 5).
Influence of Azadirachta indica leaf extracts on tumor necrosis factor-α and interleukin-6 in albino rats and its computational analysis.
Cell Mol Biol (Noisy-le-grand)
November 2024
The current study was designed to investigate the effect of A. indica (Neem) leaf extracts (ethanolic and aqueous) in yeast-induced pyrexia and acetic acid-induced writhing in rat models to evaluate the antipyretic and analgesic biomarkers and its phytochemical screening with computational analysis. For the antipyretic activity model 60 albino rats (160-200g) of either sex were divided into 4 groups and all groups were injected with yeast to induce pyrexia.
View Article and Find Full Text PDFEco-friendly green synthesis of Fe-doped WS using neem leaf extract: unlocking large interlayer spacing for improved capacitance and rapid ion transport.
RSC Adv
December 2024
Department of Physics, College of Science, University of Bisha P.O. Box 551 Bisha 61922 Saudi Arabia.
Iron-doped tungsten disulfide (Fe-WS) nanoparticles were synthesized a green method using neem leaf extract. X-ray diffraction (XRD) confirmed structural changes, with the formation of a hexagonal structure. The -spacing is increased by Fe doping (6.
View Article and Find Full Text PDFPhytochemical Analysis, Cytotoxicity, and Antitrypanosomal, Antioxidant, and Anti-Inflammatory Activities of Fruit, Leaf, and Stem Bark Extracts.
J Parasitol Res
November 2024
Plant Development Department, Centre for Plant Medicine Research, Mampong-Akuapem, Ghana.
Article Synopsis
- Human African trypanosomiasis (HAT) is a serious disease that affects both humans and animals, causing severe health issues, and this study explores the medicinal properties of plant extracts used to treat it.
- The ethanol extracts from fruit (CFE), neem leaves (NLE), and stem bark (NSBE) showed significant antitrypanosomal activity, with CFE being the most effective in inhibiting the parasite, while all extracts were non-toxic to liver cells.
- Despite NSBE having the highest antioxidant and anti-inflammatory properties, the study found that CFE and NLE have different mechanisms leading to their superior antitrypanosomal effects, leading to ongoing research on CFE’s constituents for potential new treatments
Corrigendum: The immunomodulatory impact of naturally derived neem leaf glycoprotein on the initiation progression model of 4NQO induced murine oral carcinogenesis: a preclinical study.
Front Immunol
November 2024
Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India.
[This corrects the article DOI: 10.3389/fimmu.2024.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!