Peroxiredoxins (Prxs) are a family of ubiquitous proteins that help minimize the harmful effects of oxidative stress by catalyzing the reduction of hydrogen peroxide (H2O2) and organic hydroperoxides to less harmful forms. A full-length cDNA corresponding to a 2-Cys Prx gene was isolated from the flatback mud crab Eurypanopeus depressus and designated as EdPrx-1 (GenBank accession no. EU684547). EdPrx-1 has a major open-reading frame of 594 bp and is capable of encoding a polypeptide of 198 amino acid residues. Like other 2-Cys Prxs, EdPrx-1 protein possesses two conserved cysteine residues that play an essential role for the antioxidant activity of the proteins. The EdPrx-1 protein, as deduced from the cDNA sequence, shows a high level (74-93%) of sequence similarity to the 2-Cys Prxs from other crustaceans as well as those from many arthropod species (73-76% similarity). It shares about 70% sequence similarity with homologs from mammalian species. EdPrx-1 gene is expressed at low level in the gill, hypodermis, and hepatopancreas tissues of the crab under non-stressed condition; however, its expression is elevated about three-fold in the gills under hypo-osmotic stress. This suggests a possible role in protecting against oxidative stress caused by the increased metabolic activities associated with hyperosmoregulation.
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http://dx.doi.org/10.1016/j.cbpb.2009.11.015 | DOI Listing |
Environ Microbiol
December 2023
Department of Biology, East Carolina University, Greenville, North Carolina, USA.
Growing evidence suggests that microbiomes have been shaping the evolutionary pathways of macroorganisms for millennia and that these tiny symbionts can influence, and possibly even control, species interactions like host-parasite relationships. Yet, while studies have investigated host-parasites and microbiomes separately, little has been done to understand all three groups synergistically. Here, we collected infected and uninfected Eurypanopeus depressus crab hosts from a coastal North Carolina oyster reef three times over 4 months.
View Article and Find Full Text PDFJ Invertebr Pathol
May 2022
Biology Department, East Carolina University, Greenville, NC 27858, USA.
Bacterial pathogens are a long-standing threat to the longevity and survival of crustacean hosts. Their presence and continuing emergence require close monitoring to understand their impact on fished, cultured, and wild crustacean populations. We describe a new bacterial pathogen belonging to the Anaplasmataceae family (Alphaproteobacteria: Rickettsiales), providing pathological, ultrastructural, phylogenetic, and genomic evidence to determine a candidate genus and species ('Candidatus Mellornella promiscua').
View Article and Find Full Text PDFMicrobiol Resour Announc
November 2019
Biology Department, East Carolina University, Greenville, North Carolina, USA.
A single-stranded DNA (ssDNA) virus is presented from a metagenomic data set derived from -infected hepatopancreatic tissues of the crab The circular virus genome (4,768 bp) encodes 14 hypothetical proteins, some similar to other bacteriophages (). Based on its relatedness to other , this virus represents a member of a novel genus.
View Article and Find Full Text PDFEcology
April 2017
Department of Integrative Biology, University of South Florida, 4202 East Fowler Ave, SCA 110, Tampa, Florida, 33620, USA.
Identifying and quantifying the relevant properties of habitat structure that mediate predator-prey interactions remains a persistent challenge. Most previous studies investigate effects of structural density on trophic interactions and typically quantify refuge quality using one or two-dimensional metrics. Few consider spatial arrangement of components (i.
View Article and Find Full Text PDFOecologia
April 2017
Odum School of Ecology, University of Georgia, 140 E. Green St, Athens, GA, 30602, USA.
Parasites often alter host physiology and behavior, which can enhance predation risk for infected hosts. Higher consumption of parasitized prey can in turn lead to a less parasitized prey population (the healthy herd hypothesis). Loxothylacus panopaei is a non-native castrating barnacle parasite on the mud crab Eurypanopeus depressus along the Atlantic coast.
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