AI Article Synopsis
- The study explores how melittin affects the purple membrane, highlighting its role in changing membrane color and partially regenerating the photocycle and proton pump.
- Melittin's ability to reconstitute bacteriorhodopsin is influenced by electrical charges.
- Researchers suggest that melittin binds to the membrane using both hydrophobic and electrostatic interactions, with electrostatic forces being the stronger factor, likely occurring at surface sites of the membrane.
Article Abstract
We have investigated the character of melittin-regenerated purple membrane. Adding melittin to blue membrane causes the color transition and partial regeneration of the photocycle and the proton pump. The reconstitution of bacteriorhodopsin by melittin is proved to be charge-dependent. In studying the location of melittin binding on the blue membrane, we suggest that melittin anchors on the membrane through both hydrophobic and electrostatic interactions. The electrostatic interaction is dominant. The binding sites for the electrostatic interaction should be on the surface of the membrane.
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| http://dx.doi.org/10.1134/s0006297909120128 | DOI Listing |
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