AI Article Synopsis
- A novel optical lithographic technique was used to create endothelial cell capillary networks and tested for clinical applications in treating ischemic diseases.
- Capillary structures formed on amniotic membrane scaffolds were implanted in mice, where they successfully maintained blood perfusion for at least 5 days.
- The implanted capillary networks significantly improved blood flow in ischemic limbs, reversing symptoms and enhancing mobility by restoring function comparable to nonischemic limbs within 9 days post-implantation.
Article Abstract
We previously reported a novel optical lithographic technique for the construction of a capillary network consisting of endothelial cells. To investigate the feasibility of clinical application in the treatment of ischemic diseases, capillary structures were formed on scaffolds made from amniotic membrane (AM) and implanted into mice. The capillary network remained in place for at least 5 days and blood perfusion through the implanted capillaries was histologically detected in an ear flap model. Moreover, blood was observed flowing through the capillary network implanted in abdominal subcutaneous tissue of mice at 5 days after insertion. Implantation of the AM capillary structure into the ischemic hind limbs of mice significantly increased reperfusion compared with controls (AM only). Blood flow was restored in the ischemic limbs to the level of corresponding nonischemic limbs as early as 9 days after surgical implantation. The treatment reversed ischemic symptoms, and ambulatory impairment was significantly improved. Thus, the implantation of a capillary network engineered ex vivo could have therapeutic potential for ischemic diseases.
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| http://dx.doi.org/10.1089/ten.tea.2009.0097 | DOI Listing |
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