Aim Of The Study: A Chinese herbal drug, root of Achyranthes bidentata showed a potent inhibitory activity on bone resorption induced by parathyroid hormone (PTH) in a bone organ culture using neonatal mouse parietal bones. The present study is to clarify the fractions responsible for the activity and further explore the osteoprotective effect of the fraction in vivo.
Materials And Methods: The hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH) and water soluble fractions of methanol extract of the root of Achyranthes bidentata were prepared and screened for their anti-bone resorption activity using the bone organ culture system. The n-BuOH soluble fraction was further administered orally at doses of 25, 50 and 100mg/(kgday) to ovariectomized (OVX) rats. The analyses of the rat body weight, serum estradiol (E2), total cholesterol and triglyceride levels, uteri weight and measurement of bone mineral density (BMD) were conducted.
Results: The EtOAc and n-BuOH fractions showed the most potent inhibitory activity on PTH-induced bone resorption. Further research using OVX rat model revealed that the n-BuOH fraction significantly prevented BMD loss due to OVX operation. While, the uteri weight and serum estradiol (E2), total cholesterol and triglyceride levels displayed no differences compared with those of control group (OVX rats), suggesting the n-BuOH fraction should have no estrogen-like side effects.
Conclusions: The results reveal that the n-BuOH soluble fraction of the root of Achyranthes bidentata is effective at preventing bone loss in OVX rats and has a great potential as an alternative tool for the treatment of osteoporosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2009.11.016 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The synergistic protective effects of paeoniflorin and β-ecdysterone against cardiac hypertrophy through suppressing oxidative stress and ferroptosis.
Cell Signal
January 2025
School of Basic Medicine, Qiqihar Medical University, Qiqihar 161006, China; Heilongjiang Key Laboratory of Medicine and Food Resources and Metabolic Disease Prevention, Qiqihar Medical University, Qiqihar 161006, China. Electronic address:
Exploring feasible drugs for the treatment of pathological cardiac hypertrophy has always been a focus of cardiovascular disease research. Paeoniflorin (PF) and β-Ecdysterone (β-Ecd) are the main active components of Paeonia lactiflora and Achyranthes bidentata, which can be used for the treatment of cardiovascular diseases, but their mechanism of action remains unclear. This study focused on oxidative stress and ferroptosis to investigate the protective effects of PF and β-Ecd on cardiac hypertrophy in primary cardiomyocytes and C57BL/6 mice, utilizing the integration of CCK8 assays, ROS detection, molecular docking, real-time quantitative PCR, western blot, immunofluorescence, etc.
View Article and Find Full Text PDFEffect of microplastics on the allelopathic effects of native and invasive plants on co-occurring invaders.
Front Plant Sci
October 2024
School of Advanced Study, Taizhou University, Taizhou, Zhejiang, China.
Introduction: Microplastic pollution has emerged as a significant global change factor, with the potential to alter the biological, physicochemical properties of soil and to subsequently affect plant growth. Despite growing recognition of the impacts of microplastic pollution, the mechanisms by which microplastics modify plant leaf chemistry and influence allelopathic interactions among co-existing plant species remain unclear.
Methods: We used the native perennial forb and the invasive annual forb as focal species.
Exploring synergistic effects of Blume and Pall. on hypertension with liver yang hyperactivity using the multidisciplinary integrative strategy.
Heliyon
November 2024
Center of Scientific Research, School of Basic Medicine, Qiqihar Medical University, Qiqihar, 161006, China.
Traditional Chinese Medicine (TCM) formulations serve as a multi-component pharmacological combination therapy with various potential targets and have collected extensive knowledge regarding the in vivo efficacy of treating cardiovascular disorders in clinical practice for thousands of years. However, the obscurity of the chemicals and the molecular mechanisms are impediments to their continued growth and globalization. Therefore, new modern medications based on the combination of beneficial TCM components with precise clinical efficacy are required.
View Article and Find Full Text PDFOsteoprotective effect of root extract on osteoporotic rats: a systematic review and meta-analysis.
Pharm Biol
December 2024
The Health Preservation and Rehabilitation College, Chengdu University of Chinese Traditional Medicine, Chengdu City, Sichuan Province, China.
Context: Blume (ABB), a plant of Amaranthaceae family, has been one of the more commonly used phytomedicine remedies for thousands of years, and recent studies have highlighted the efficacy of its extracts in the treatment of osteoporosis. Nonetheless, a thorough analysis of its benefits is currently absent.
Objective: This meta-analysis assessed the effects of ABB root extract (ABBRE) on osteoporotic rats and provides a rationale for future clinical studies.
The functional identification and evaluation of endophytic bacteria sourced from the roots of tolerant to overcome monoculture problems of .
Front Microbiol
July 2024
Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
The isolation and identification of plant growth-promoting endophytic bacteria (PGPEB) from roots have profound theoretical and practical implications in ecological agriculture, particularly as bio-inoculants to address challenges associated with continuous monoculture. Our research revealed a significant increase in the abundance of these beneficial bacteria in rhizosphere soil under prolonged monoculture conditions, as shown by bioinformatics analysis. Subsequently, we isolated 563 strains of endophytic bacteria from roots.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!