An understanding of bovine placental gene expression is essential for the study of animal reproductive physiology. Recent reports have found that placental abnormalities occur frequently in cloned bovines and mice. However, the molecular mechanisms underlying bovine placenta function remain unclear. Here, we present a preliminary description of the bovine placenta proteome. Proteins within the isoelectric point ranging from 4.0 to 7.0 and 6.0 to 9.0 were analyzed separately using 2-DE, using three replicates of bovine placenta. Approximately 2000 spots were detected in a placental 2-D gel stained with Coomassie blue. Subsequent excision of 380 spots from gels and MALDI-TOF MS analysis allowed the identification of 273 proteins. Our results revealed the composite profiles of key proteins in the bovine placenta during late pregnancy. These protein profiles will shed light on placental function during pregnancy and assist with functional analysis of the proteins.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/pmic.200900508 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Analysis of morphology, cytotoxicity, and water content characteristics of freeze-dried amnion membrane from human and bovine.
Narra J
December 2024
Department of Polymer Science and Engineering, Chonbuk National University, Jeonju, South Korea.
Placenta tissue has biological advantages, including anti-inflammatory, anti-bacterial, anti-fibrotic formation, and immunomodulatory properties. The amnion membrane (AM) is an inner side membrane of the placenta that faces the fetus. The main sources of amnion are humans and animals, with bovine being one of the significant sources.
View Article and Find Full Text PDFZoonotic Disease: Knowledge, Attitude and Practice of Dairy Farm Owner in Wolaita Sodo District, Ethiopia.
Vet Med Sci
January 2025
College of Veterinary Medicine and Animal Science, Samara University, Samara, Ethiopia.
Background: Lack of knowledge regarding zoonotic transmission, prevention and control measures is a potential high risk for the occurrence of zoonotic diseases.
Objective: The study aimed to assess knowledge, attitude and practices of dairy farm participants concerning zoonoses.
Animals: A cross-sectional study was conducted from March to August 2022 in and around Sodo town, using a questionnaire among dairy farm participants (n = 123).
Prevalence of chlamydiae in dairy cattle herds and factors contributing to the spread of infections.
J Vet Res
December 2024
Department of Cattle and Sheep Diseases, Puławy, Poland.
Introduction: Different species affect cattle and contribute to economic losses. One of them, , is a globally endemic livestock pathogen. Despite its endemicity, prevalence data from Poland have so far been limited.
View Article and Find Full Text PDFA Case-Control Study on the Usefulness of Serum Lecithin: Cholesterol Acyltransferase Activity as a Predictor of Retained Placenta in Close-Up Dairy Cows.
Animals (Basel)
December 2024
Veterinary Herd Health, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Hokkaido, Japan.
The purpose of this study was to investigate the usefulness of the activity of lecithin:cholesterol acyltransferase (LCAT), the enzyme responsible for esterification of cholesterol in plasma, as a predictor of retained placenta (RP) in close-up cows, compared with the non-esterified fatty acids (NEFA) concentration. This study was conducted as a case-control study between February 2010 and February 2016, on a single farm with approximately 200 Holstein parous cows in Hokkaido, Japan. Of the 1187 dairy cattle that calved, 835 dairy cattle were enrolled that underwent routine regular health examinations including blood sampling, body condition score (BCS) and the rumen fill score (RFS) at the close-up stage between 2 and 21 days before their expected calving dates.
View Article and Find Full Text PDFOptimization of a Conventional Assay into SYBR Green Real-Time PCR for the Detection of the Nc5 Segment from Neospora caninum.
Acta Parasitol
January 2025
Department of Veterinary Medicine, Federal University of Paraná, Rua Dos Funcionários, 1540, Curitiba, Paraná, 80035-050, Brazil.
Purpose: The aim of the present study was to establish a SYBR Green-based real-time PCR assay for detection of the Nc5 segment from the Neospora caninum genome.
Methods: The oligonucleotides sequences targeting the Nc5 gene previously reported and designed in-house were validated. Two Primer sets were evaluated and tested in four different combinations.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!