AI Article Synopsis

  • Met4 is a key transcriptional activator for sulfur metabolism in yeast, but it needs to work with specific cofactors since it can’t bind DNA on its own.
  • Researchers deleted different cofactors to see how they affect Met4's ability to activate genes, identifying 45 core target genes essential for the metabolic process.
  • The study found that Met31 and Met32 are crucial for activating all core genes, while Met28 and Cbf1 influence only certain genes, indicating complex interactions and varying binding behaviors among these cofactors based on specific conditions.

Article Abstract

Met4 is the transcriptional activator of the sulfur metabolic network in Saccharomyces cerevisiae. Lacking DNA-binding ability, Met4 must interact with proteins called Met4 cofactors to target promoters for transcription. Two types of DNA-binding cofactors (Cbf1 and Met31/Met32) recruit Met4 to promoters and one cofactor (Met28) stabilizes the DNA-bound Met4 complexes. To dissect this combinatorial system, we systematically deleted each category of cofactor(s) and analyzed Met4-activated transcription on a genome-wide scale. We defined a core regulon for Met4, consisting of 45 target genes. Deletion of both Met31 and Met32 eliminated activation of the core regulon, whereas loss of Met28 or Cbf1 interfered with only a subset of targets that map to distinct sectors of the sulfur metabolic network. These transcriptional dependencies roughly correlated with the presence of Cbf1 promoter motifs. Quantitative analysis of in vivo promoter binding properties indicated varying levels of cooperativity and interdependency exists between members of this combinatorial system. Cbf1 was the only cofactor to remain fully bound to target promoters under all conditions, whereas other factors exhibited different degrees of regulated binding in a promoter-specific fashion. Taken together, Met4 cofactors use a variety of mechanisms to allow differential transcription of target genes in response to various cues.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2814790PMC
http://dx.doi.org/10.1091/mbc.e09-05-0420DOI Listing

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