AI Article Synopsis
- The study aimed to analyze how the levels of alkaline and acid components in Fuzi and Gancao changed when combined in different ways in Sini Decotion.
- The researchers used HPLC to measure the amounts of aconite alkaloids and glycyrrhizic acid in various extracts, including those of Fuzi, Gancao, both combined, and the complete recipe.
- Results showed that the quantity of aconite alkaloids and glycyrrhizic acid decreased when moving from single medicines to combinations, with changes being more significant in water extracts for Fuzi and across all combinations for Gancao.
Article Abstract
Objective: To determine the change pattern of alkaline and acid components of Fuzi and gancao amoung different combinations in Sini Decotion.
Method: The contents of aconite alkaloids and glycyrrhizic acid were determined by HPLC in samples of Fuzi extracts, gancao extracts, Fuzi and gancao pair medicines extracts, and extracts of whole recipe.
Result: As for Fuzi, monoester aconite alkaloids could be detected in water extracts, and both biester and monoester aconite alkaloids could be detected in ethanol extracts. The contents of aconite alkaloids were decreased with changes of combinations: from Fuzi alone to pair medicines, and to whole recipe. The change pattern was more apparent when extracted in water decoction. As for Gancao, the glycyrrhizic acid was lowered from single medicine to pair medicines, and to whole recipe.
Conclusion: The contents of alkaline and acid components were changed with different extract methods and different combinations of Fuzi and Gancao.
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