To optimize program of bovine somatic nuclear transfer, we used two different enucleation procedures (by Spindle-view system & Hoechst 33342 staining), two different procedures to introduce donor nuclei (by ooplasm microinjection & electrofusion), and three different group electrofusion parameters (group 1: 1.9 kV/cm, 10 micros, two; group 2: 1.5 kV/cm, 25 micros, two; group 3: 0.6 kV/cm, 100 micros, one) to reconstruct bovine cloned embryos. The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 micros, two) to reconstruct bovine cloned embryos. Then the excellent blastocysts were transferred to fosters for producing cloned cattle 80 high-quality cloned blastocysts were transferred into 33 fosters, two cloned calves were produced. According to the results, the optimized program could be used to produce cloned cattle.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Pulsed Electric Field (PEF) Treatment Results in Growth Promotion, Main Flavonoids Extraction, and Phytochemical Profile Modulation of Georgi Roots.
Int J Mol Sci
December 2024
Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biology and Botany, Wrocław Medical University, 50-367 Wrocław, Poland.
This study aims to explore the effect of pulsed electric field (PEF) treatment as a method very likely to result in reversible electroporation of Georgi underground organs, resulting in increased mass transfer and secondary metabolites leakage. PEF treatment with previously established empirically tailored parameters [E = 0.3 kV/cm (U = 3 kV, d = 10 cm), t = 50 µs, N = 33 f = 1 Hz] was applied 1-3 times to roots submerged in four different Natural Deep Eutectic Solvents (NADES) media (1-choline chloride/xylose (1:2) + 30% water, 2-choline chloride/glucose (1:2) + 30% water, 3-choline chloride/ethylene glycol (1:2), and 4-tap water (EC = 0.
View Article and Find Full Text PDFSupercritical fluid extraction prior pulsed electric fields to improve high-added-value compounds recovery from salmon side streams: Evaluation of protein, bioactive peptides and minerals.
Food Chem
March 2025
Research group in Innovative Technologies for Sustainable Food (ALISOST), Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, 46100, Burjassot, València, Spain.
A sequential extraction process combining supercritical fluid extraction (SFE: 25.0 MPa, 75 min, 50.0 °C, and 10.
View Article and Find Full Text PDFEnhancing lung cancer growth inhibition with calcium ions: Role of mid- and high-frequency electric field pulses.
Biomed Pharmacother
December 2024
Wroclaw Medical University, Faculty of Pharmacy, Department of Molecular and Cellular Biology, Wroclaw, Poland; Medical University Hospital, Wroclaw, Poland. Electronic address:
Calcium electroporation (CaEP) involves the combination of calcium ions with electroporation, which is induced by pulsed electric fields (PEFs). This study explores the application of high-frequency unipolar nanosecond pulsed electric fields (nsPEFs: 8-14 kV/cm, 200 ns, 10 kHz, 100 kHz, 1 MHz repetition frequency pulse bursts, n = 100) and their potential in inhibiting lung cancer cell growth. As a reference, standard microsecond range parametric protocols were used (100 µs x 8 pulses).
View Article and Find Full Text PDFSince the recycling of composites from plant tissues is difficult, extraction of bioactive compounds from plant sources requires pre-treatment by new technology such as pulsed electric fields (PEF). Due to the reduced consumption of organic solvents, the extractive techniques such as using supercritical CO (SC-CO) are of interest to researchers. This work aimed to investigating the influences of different parameters of SC-CO (pressure, modifier volume, temperature, and dynamic time) and PEF (frequency and field strength) treatments on the amount extraction of β-carotene, lycopene, lutein, β-cryptoxanthin, total phenol content (TPC), and also antioxidant activity percentage of tomato to obtain the optimum circumstances extraction via PEF and SC-CO methods.
View Article and Find Full Text PDFUnveiling the synergistic effect of octenyl succinic anhydride and pulsed electric field on starch nanoparticles.
Int J Biol Macromol
September 2024
Department of Food Engineering and Technology, SLIET, Punjab, India. Electronic address:
In this study, guinea starch nanoparticles (GSNP) were prepared by nanoprecipitation technique and modified with octenyl succinic anhydride (3 %) and pulsed electric field (1.5, 3.0, and 4.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!