This paper discusses the different analysis methods used in holographic particle image velocimetry to measure particle displacement and compares their relative performance. A digital holographic microscope is described and is used to record the light scattered by particles deposited on cover slides that are displaced between exposures. In this way, particle position and displacement are controlled and a numerical data set is generated. Data extraction using nearest neighbor analysis and correlation of either the reconstructed complex amplitude or intensity fields is then investigated.
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http://dx.doi.org/10.1364/AO.48.006400 | DOI Listing |
Beilstein J Nanotechnol
December 2024
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent'ev av., 8, Novosibirsk, 630090, Russian Federation.
A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural protein corona on natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix the natural corona on "biological nanoparticles" (bio-NPs) in fetal bovine serum and newborn bovine serum; native sera served as a control. To isolate photomodified bio-NPs, we used ultracentrifugation (UC), sucrose gradient (12%, 30%, and 50%), and sucrose cushion (30%) methods.
View Article and Find Full Text PDFCardiovasc Eng Technol
January 2025
Department of Cardiac Surgery, Heidelberg University Hospital, Heidelberg, Germany.
The flow convergence method includes calculation of the proximal isovelocity surface area (PISA) and is widely used to classify mitral regurgitation (MR) with echocardiography. It constitutes a primary decision factor for determination of treatment and should therefore be a robust quantification method. However, it is known for its tendency to underestimate MR and its dependence on user expertise.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Advanced Science and Technology, Toyota Technological Institute, 2-12-1 Hisakata, Tempaku-Ku, Nagoya, Aichi, 468-8511, Japan.
In this study, a new technique for active control of the flow around a corner is proposed and a key parameter dominating the flow deflection angle is proposed. In the technique, a microjet array is used for controlling the deflection of the flow at 33 m/s ~ 54 m/s around the 25-degree corner with a small downstream-facing step, the surface of which is lined with the micro-orifices from which jets are injected into the flow. The flow velocities around the corner are measured using a PIV (particle image velocimetry) technique under each condition for injecting the microjets into the flow.
View Article and Find Full Text PDFInt J Radiat Oncol Biol Phys
January 2025
Department of Radiation Oncology, University of Florida College of Medicine, Jacksonville, FL, USA.
Objectives: Radiotherapy manages pancreatic cancer in various settings; however, the proximity of gastrointestinal (GI) luminal organs-at-risk (OAR) poses challenges to conventional radiotherapy. Proton beam therapy (PBT) may reduce toxicities compared to photon therapy. This consensus statement summarizes PBT's safe and optimal delivery for pancreatic tumors.
View Article and Find Full Text PDFJ Vis Exp
December 2024
Department of Medicine, New Jersey Medical School, Rutgers - The State University of New Jersey;
Fungi infect humans when environmental spores are inhaled into the lungs. The lung is a heterogeneous organ. Conducting airways, including bronchi and bronchioles, branch until terminating in the alveolar airspace where gas exchange occurs.
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