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Sequence-level comparative analysis of the Brassica napus genome around two stearoyl-ACP desaturase loci. | LitMetric

AI Article Synopsis

  • The study compared the genomes of Brassica napus (oilseed rape), Brassica rapa, and Arabidopsis thaliana by analyzing complete bacterial artificial chromosome (BAC) clones.
  • A new BAC library for Brassica napus was created, and genes related to fat metabolism were sequenced to assess conservation among species.
  • Findings showed only 56% gene conservation between Brassica napus and Arabidopsis thaliana, which is lower than previously reported, and indicated a greater divergence between B. napus and B. rapa genomes than expected.

Article Abstract

We conducted a sequence-level comparative analyses, at the scale of complete bacterial artificial chromosome (BAC) clones, between the genome of the most economically important Brassica species, Brassica napus (oilseed rape), and those of Brassica rapa, the genome of which is currently being sequenced, and Arabidopsis thaliana. We constructed a new B. napus BAC library and identified and sequenced clones that contain homoeologous regions of the genome including stearoyl-ACP desaturase-encoding genes. We sequenced the orthologous region of the genome of B. rapa and conducted comparative analyses between the Brassica sequences and those of the orthologous region of the genome of A. thaliana. The proportion of genes conserved (approximately 56%) is lower than has been reported previously between A. thaliana and Brassica (approximately 66%). The gene models for sets of conserved genes were used to determine the extent of nucleotide conservation of coding regions. This was found to be 84.2 +/- 3.9% and 85.8 +/- 3.7% between the B. napus A and C genomes, respectively, and that of A. thaliana, which is consistent with previous results for other Brassica species, and 97.5 +/- 3.1% between the B. napus A genome and B. rapa, and 93.1 +/- 4.9% between the B. napus C genome and B. rapa. The divergence of the B. napus genes from the A genome and the B. rapa genes was greater than anticipated and indicates that the A genome ancestor of the B. napus cultivar studied was relatively distantly related to the cultivar of B. rapa selected for genome sequencing.

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Source
http://dx.doi.org/10.1111/j.1365-313X.2009.04084.xDOI Listing

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