AI Article Synopsis
- - Tubulin polymerization in a restricted environment and temperature gradient leads to the formation of organized microtubule structures with a specific directional preference.
- - The study employed three microscopy techniques to investigate the microtubule assemblies at different scales: polarized light microscopy for larger structures, fluorescence microscopy for medium-scale analysis, and transmission electron microscopy for finer details.
- - The research highlights the importance of conditions like confinement and temperature in influencing the alignment and properties of microtubules, which are crucial for various biological functions.
Article Abstract
Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).
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| http://dx.doi.org/10.1021/ja901538n | DOI Listing |
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