Serological screening of sera from orang-utans demonstrated a high percentage of sera that cross-reacted with antigens of the polyomavirus (PyV) simian virus 40. Analysis of archival DNA samples from 71 Bornean and eight Sumatran orang-utans with a broad-spectrum PCR assay resulted in the detection of PyV infections in 11 animals from both species. Sequence analysis of the amplicons revealed considerable differences between the PyVs from Bornean and Sumatran orang-utans. The genome from two PyVs, one from each species, was therefore amplified and sequenced. Both viral genomes revealed a characteristic PyV architecture, but lacked an obvious agnogene. Neighbour-joining analysis positioned the viruses in a large cluster together with viruses from bats, bovines, rodents and several primate PyVs from chimpanzees, African green monkeys, squirrel monkeys and the human Merkel cell PyV.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/vir.0.017673-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Non-canonical DNA in human and other ape telomere-to-telomere genomes.
bioRxiv
December 2024
Department of Biology, Penn State University, University Park, PA 16802, USA.
Non-canonical (non-B) DNA structures-e.g., bent DNA, hairpins, G-quadruplexes, Z-DNA, etc.
View Article and Find Full Text PDFEvolutionary Dynamics of G-Quadruplexes in Human and Other Great Ape Telomere-to-Telomere Genomes.
bioRxiv
November 2024
Department of Biology, Penn State University, University Park, PA 16802, USA.
G-quadruplexes (G4s) are non-canonical DNA structures that can form at approximately 1% of the human genome. G4s contribute to point mutations and structural variation and thus facilitate genomic instability. They play important roles in regulating replication, transcription, and telomere maintenance, and some of them evolve under purifying selection.
View Article and Find Full Text PDFSpecies Identification of Rehabilitated Critically Endangered Orangutans Through DNA Forensic: Implication for Conservation.
Trop Life Sci Res
March 2024
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sinduadi. Mlati, Sleman, 55281. Special Region of Yogyakarta, Indonesia.
Rehabilitating and releasing orangutans back into the wild is one of the conservation strategies being pursued to conserve orangutans. However, the species determination between Sumatran, Tapanuli, and Bornean orangutans is essential for reintroduction to avoid outbreeding depression, which could lead to DNA hybridisation and increase the probability of recessive characters. Here, we reported on an investigation of three orangutans in which DNA forensic techniques were used to identify the species before release and reintroduction to their habitat.
View Article and Find Full Text PDFComplete sequencing of ape genomes.
bioRxiv
October 2024
Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.
Article Synopsis
- The study presents detailed genomes of six ape species, achieving high accuracy and complete sequencing of all their chromosomes.
- It addresses complex genomic regions, leading to enhanced understanding of evolutionary relationships among these species.
- The findings will serve as a crucial resource for future research on human evolution and our closest ape relatives.
The complete sequence and comparative analysis of ape sex chromosomes.
Nature
June 2024
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.
Article Synopsis
- Apes have two sex chromosomes: the essential Y chromosome for male reproduction and the X chromosome necessary for both reproduction and cognition, with differences in mating patterns affecting their function.
- Studying these chromosomes is challenging due to their repetitive structures, but researchers created gapless assemblies for five great apes and one lesser ape to explore their evolutionary complexities.
- The Y chromosomes are highly variable and undergo significant changes compared to the more stable X chromosomes, and this research can provide insights into human evolution and aid in the conservation of endangered ape species.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!