Direct electrochemistry and activity of myoglobin (Mb) immobilized on carbon nanotube (CNT) forest electrodes were investigated by probing mainly its electrocatalytical response towards oxygen. The protein was anchored on the CNT electrodes through carbodiimide coupling, which was shown to provide long term stability. The electrochemical response was monitored as a function of oxygen concentration and pH. Conformational changes together with the consequent loss of oxygen affinity were recorded at low pH, which delimits the operative range of the Mb/CNT electrodes for sensing purposes. In general, it can be concluded that CNT forests constitute suitable platforms for Mb attachment without compromising the protein bioactivity and by keeping at the same time the direct electron exchange with the heme core. All these characteristics confer to the protein modified CNT system promising properties for the implementation of (bio)sensor devices with impact in the clinical and environmental field.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1166/jnn.2009.1566 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An electrochemiluminescence biosensor based on silver-cysteine nanorod as an emitter and AgNP-decorated FeMoO as a signal amplifier for sensitive detection of heart-type fatty acid binding protein.
Mikrochim Acta
December 2024
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China.
An electrochemiluminescence (ECL) immunosensor was developed for the highly sensitive and specific detection of heart-type fatty acid binding protein (H-FABP) and the rapid diagnosis of acute myocardial infarction (AMI). H-FABP is a biomarker that is highly specific to cardiac tissue and is associated with a range of cardiac diseases. Following myocardial injury, the rate of increase in H-FABP levels is greater than that observed for myoglobin and troponin.
View Article and Find Full Text PDFSex similarities and divergences in systemic and muscle iron metabolism adaptations to extreme physical inactivity in rats.
J Cachexia Sarcopenia Muscle
October 2024
Laboratory "Movement Sport and Health Sciences" EA7470, University of Rennes/ENS, Rennes, France.
Background: Previous data in humans suggest that extreme physical inactivity (EPI) affects iron metabolism differently between sexes. Our objective was to deepen the underlying mechanisms by studying rats of both sexes exposed to hindlimb unloading (HU), the reference experimental model mimicking EPI.
Methods: Eight-week-old male and female Wistar rats were assigned to control (CTL) or hindlimb unloading (HU) conditions (n = 12/group).
Rapid and sensitive detection of heart-type fatty acid binding protein using aggregation-induced emission nanoparticles on digital microfluidics workstation.
Biosens Bioelectron
October 2024
Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, China; Medical Big Data Center, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong Province, 510080, China. Electronic address:
Early and rapid diagnostic of acute myocardial infarction (AMI) during its developing stage is crucial due to its high fatality rate. Heart-type fatty acid binding protein (h-FABP) is an ideal biomarker for the quantitative diagnosis of AMI, surpassing traditional markers such as myoglobin, creatine phosphokinase-MB, and troponin in terms of sensitivity, specificity, and prognostic value. To obtain diagnostic and prognostic information, a precise and fully quantitative measurement of h-FABP is essential, typically achieved through an immunosorbent assay like the enzyme-linked immunosorbent assay.
View Article and Find Full Text PDFSupramolecular Polymer Frameworks with Controlled and Uniform Pore Apertures.
Angew Chem Int Ed Engl
September 2024
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, China.
Porous frameworks with controlled pore structure and tunable aperture are greatly demanded. However, precise synthesis of this kind of materials is a formidable challenge. Herein, we report the fabrication of two-dimensional (2D) supramolecular polymer frameworks using a precisely synthesized rod-like helical polyisocyanide as link.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!