The objective of this study was to analyze the immunogenicity and antigenicity of the V3 domain (Cys313-Cys346) of the external envelope glycoprotein gp125 of SIVmac251. The corresponding peptide was synthesized and characterized as linear and cyclic peptides. Our results showed that this region, as for HIV-1, contained an immunodominant epitope. The antigenicity was similar for the linear and cyclic peptides when tested against a panel of 15 sera from SIV infected macaques. Similarly, both peptide structures presented similar immunogenicity as shown by the characterization of the anti-peptide antibodies produced in rabbits against the cyclic and linear forms. But, unexpectedly, the antibodies produced against linear peptides recognized with a relatively higher intensity the native envelope gp140 than those produced against the cyclic structure. Furthermore, we showed that these antibodies recognized better the deglycosylated form of the glycoprotein. But, in contrast to the neutralizing activity obtained with anti-V3 peptides from HIV-1, no antiviral activity was obtained with antibodies generated against linear or cyclic SIVmac V3 peptides.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/psc.1193 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effective prediction of organosilicon molecular structures and risks in aquatic environment with machine learning.
Sci Total Environ
January 2025
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 330106, China.
Until now, mass spectrometry databases lack molecular information of most organosilicon oligomers, and risk models needing accurate molecular descriptors are unavailable for these emerging contaminants with thousands of monomers. To address this issue, based on molecular/fragment ions and relative abundance from GC-Orbitrap-MS, this study developed appropriate classification (accuracies = 0.750-0.
View Article and Find Full Text PDFA novel electrochemical sensor for the determination of cadmium ions based on nitrogen-enriched carbon modified electrode.
Sci Rep
January 2025
Department of Nanotechnology, Faculty of Chemistry, Urmia University, Urmia, Iran.
In the present work, nitrogen-doped carbon was synthesized starting from a chitosan/urea mixture and immobilized at the surface of a bare glassy carbon electrode to detect Cd(II) ions using differential pulse-anodic stripping voltammetry method (DP-ASV). The synthesized nitrogen-doped carbon showed a significant potential for determining Cd(II) ions. Doping carbon with nitrogen atoms gives a structure with increased valence band energy, leading to acceleration of the electron transfer by creating an interaction of nitrogen's free electrons with Cd(II), which subsequently increases the peak current value.
View Article and Find Full Text PDFCharacterization and assessment of hydrogen leakage mechanisms in salt caverns.
Sci Rep
January 2025
Department of Energy Resources, University of Stavanger, Stavanger, Norway.
Salt caverns are widely regarded as a suitable option for the underground storage of hydrogen. However, an accurate assessment of the hydrogen leakage through the walls of salt caverns into the surrounding formations remains crucial. In this work, the flow of hydrogen into the surrounding formation is evaluated by assuming that salt rock consists of bundles of tortuous nano-capillary tubes.
View Article and Find Full Text PDFAscorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples.
Sci Rep
January 2025
Department of Biochemistry, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Liver cancer is globally the most frequent fatal malignancy, and its identification is critical for making clinical decisions about treatment options. Pathological diagnostics and contemporary imaging technologies provide insufficient information for tumor identification. Hydrogen peroxide (HO), an emerging biomarker is a powerful oxidant found in the tumor microenvironment, and stimulates the invasion, proliferation, and metastasis of liver cancer cells.
View Article and Find Full Text PDFPrognosis of Cardiovascular Conditions Noninvasively Using Printable Elastomeric Electronic Skin.
Adv Healthc Mater
January 2025
Rubber Technology Centre, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Lack of timely prognosis of cardiovascular condition (CVC) is resulting in increased mortality across the globe. Currently, available techniques are confined to medical facilities and need the intervention of specialists. Frequently, this impedes timely treatment, driven by socioeconomic factors.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!