AI Article Synopsis
- A study developed an IgG-ELISA and a rapid flow-through enzyme immunoassay to efficiently screen for leptospiral antibodies in dogs, using the recombinant LipL41 protein as the antigen.
- Comparative results indicated that the IgG-ELISA had a sensitivity of 75.46% and specificity of 93.29%, while the flow-through assay showed higher sensitivity at 87.73% and slightly lower specificity at 89.63%.
- Ultimately, the flow-through-based dot-immunobinding assay was determined to be a valid and reliable screening method for canine leptospirosis.
Article Abstract
An IgG-ELISA used recombinant antigen and a rapid flow-through enzyme immunoassay were developed for rapid screening of leptospiral antibodies in dogs using recombinant LipL41, which is one of the conserved outer membrane proteins in pathogenic leptospires as the coating antigen. Results from this study were compared with the standard microscopic agglutination test and found that the sensitivity and specificity of the enzyme-linked immunosorbent assay were 75.46% and 93.29% and whereas that of flow-through-based dot-immunobinding assay were 87.73% and 89.63%, respectively. Relative merits of these tests were also assessed. The flow-through-based dot-immunobinding assay was thus proved to be a valid screening test for canine leptospirosis.
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| http://dx.doi.org/10.1016/j.cimid.2009.10.003 | DOI Listing |
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Development of rapid flow-through-based dot-immunoassay for serodiagnosis of leptospirosis in dogs.
Comp Immunol Microbiol Infect Dis
January 2011
Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai, India.
Article Synopsis
- A study developed an IgG-ELISA and a rapid flow-through enzyme immunoassay to efficiently screen for leptospiral antibodies in dogs, using the recombinant LipL41 protein as the antigen.
- Comparative results indicated that the IgG-ELISA had a sensitivity of 75.46% and specificity of 93.29%, while the flow-through assay showed higher sensitivity at 87.73% and slightly lower specificity at 89.63%.
- Ultimately, the flow-through-based dot-immunobinding assay was determined to be a valid and reliable screening method for canine leptospirosis.
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