We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from alpha1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ac902014c | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cellular EMT-status governs contact guidance in an electrospun TACS-mimicking model.
Mater Today Bio
February 2025
Pharmaceutical Technology and Biopharmaceutics, Department of Pharmacy, Ludwig-Maximilians-University München, Munich, Germany.
In this study, an advanced nanofiber breast cancer model was developed and systematically characterized including physico-chemical, cell-biological and biophysical parameters. Using electrospinning, the architecture of tumor-associated collagen signatures (TACS5 and TACS6) was mimicked. By employing a rotating cylinder or static plate collector set-up, aligned fibers (TACS5-like structures) and randomly orientated fibers (TACS6-like structures) fibers were produced, respectively.
View Article and Find Full Text PDFFacile universal strategy of presenting multifunctional short peptides for customizing desired surfaces.
J Nanobiotechnology
January 2025
Department of Spinal Surgery, The First People's Hospital of Wenling, Affiliated Wenling Hospital, Wenzhou Medical University, Taizhou, Zhejiang, 317500, China.
Article Synopsis
- Interfacial properties of biomaterials influence critical functions like cell adhesion and tissue repair, making their manipulation essential for clinical applications.
- The study develops a versatile layer-by-layer (LbL) strategy to effectively attach peptides to substrates using polyphenols, enhancing interfacial functionalities.
- The resulting peptide-polyphenol coatings demonstrate broad applicability, stability, and the ability to incorporate various functional molecules for improved biomaterial performance.
Magnetically Induced Anisotropic Microstructures on Polyethylene Glycol Hydrogel Facilitate BMSC Alignment and Osteogenic Differentiation.
Gels
December 2024
Research Institute of Smart Medicine and Biological Engineering, Health Science Center, Ningbo University, Ningbo 315211, China.
Many tissues exhibit structural anisotropy, which imparts orientation-specific properties and functions. However, recapitulating the cellular patterns found in anisotropic tissues presents a remarkable challenge, particularly when using soft and wet hydrogels. Herein, we develop self-assembled anisotropic magnetic FeO micropatterns on polyethylene glycol hydrogels utilizing dipole-dipole interactions.
View Article and Find Full Text PDFAmphiphilic Marine Antifouling Coatings Based on Zwitterion-Modified Silicone Polymers.
Langmuir
December 2024
Department of Biochemical Engineering, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
Silicone coatings are widely employed in marine antifouling applications due to their low surface energy. However, in static marine environments, pure silicone coatings are ineffective in preventing the adhesion of marine biofilms, which consist of proteins, marine bacteria, and extracellular matrices, ultimately promoting the attachment of macrofouling organisms. To address the limitations in antifouling performance under static conditions, this study introduces a silicone-based antifouling coating modified with zwitterionic polymers.
View Article and Find Full Text PDFComparative study of crystallization kinetics and phase segregation of triple cation and methylammonium lead iodide perovskites on moisture probing using synchrotron X-ray based radiation.
J Synchrotron Radiat
January 2025
Department of Physics, Masinde Muliro University of Science and Technology, Kakamega, Kenya.
3D mixed perovskites have achieved substantial success in boosting solar cell efficiency, but the complicated perovskite crystal formation pathway remains mysterious. Here we present detailed crystallization kinetics of mixed perovskites FAMAPb(IBr), where FA is formamidinium and MA is methylammonium, with the addition of Cs to form a triple cation perovskite (3-CAT), in a comparison with the perovskite building block MAPbI (MAPI) via static grazing-incidence wide-angle X-ray scattering (GIWAXS) and micro-diffraction measurements. Spin-coated films produced α-perovskite peaks with no PbI or δ-intermediate phases, which was a promising result for the 3-CAT perovskite from micro-diffraction measurements.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!